Identification of Alternaria, Aspergillus, Fusarium and Penicillium spp. on Onion Plant (Allium cepa L.) Growing in Hatay, Amasya and Tokat Provinces Using MALDI-TOF Mass Spectrometry

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dc.contributor.authorKara, Merve
dc.contributor.authorSoylu, Emine Mine
dc.date.accessioned2024-09-19T16:22:23Z
dc.date.available2024-09-19T16:22:23Z
dc.date.issued2023
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractPlant fungal disease pathogens cause significant yield and quality losses in onion growing areas. In addition to yield losses, they cause negative effects that reduce the quality and export potential of the product, resulting in significant economic losses during harvest, post-harvest, processing and marketing stages. In recent years, Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) has emerged as a rapid, cost-effective, reproducible, and powerful technique for identifying microorganisms, and its impact on microbiological diagnosis has transformed workflow in equipped laboratories. In this study, proteomic analyzes were performed on Alternaria, Aspergillus, Fusarium and Penicillium species isolated from onion growing areas in Hatay, Amasya, and Tokat provinces. After extraction of mycelium from single spore cultures of the isolates with ethanol-formic acid, the spectra of the individual fungal isolates were determined using the Flex control software program. These spectra were compared with Maldi Biotyper Real-Time Classification (RTC) and identification was performed. Of 519 different fungal isolates, 435 representative fungal isolates (83.8%) were identified by MALDI TOF MS. Eighty- four fungal isolates could not be identified because they were not in a satisfactory range of purity and identification. Of the 435 isolates, 269 (61.8%) were identified as Fusarium spp., 80 isolates (18.4%) were identified as Alternaria spp., 60 isolates (13.8%) as Aspergillus spp., and 26 isolates (6.0%) as Penicillium spp. Among the fungal isolates, 72.5% of the Fusarium isolates, 78.8% of the Alternaria isolates, 90.0% of the Aspergillus isolates and 84.6% of the Penicillium isolates were identified as “highly probable” species with score values between 2.000-3.000 (green color). These species are Alternaria alternata, Alternaria infectoria, Aspergillus flavus, Aspergillus niger, Fusarium culmorum, Fusarium moniliforme, Fusarium oxysporum, Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Penicillium commune and Penicillium glabrum. The results clearly demonstrate that MALDI TOF MS biotyping may be used as a highly reliable and economical diagnostic method for routine diagnosis of diseases caused by Alternaria, Aspergillus, Fusarium and Penicillium species.en_US
dc.identifier.doi10.24925/turjaf.v11is1.2525-2529.6420
dc.identifier.endpage2529en_US
dc.identifier.issn2148-127X
dc.identifier.issueS1en_US
dc.identifier.startpage2525en_US
dc.identifier.trdizinid1256495en_US
dc.identifier.urihttps://doi.org/10.24925/turjaf.v11is1.2525-2529.6420
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1256495
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15813
dc.identifier.volume11en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectOnionen_US
dc.subjectDisease pathogenen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectDiagnosisen_US
dc.subjectProteomicen_US
dc.titleIdentification of Alternaria, Aspergillus, Fusarium and Penicillium spp. on Onion Plant (Allium cepa L.) Growing in Hatay, Amasya and Tokat Provinces Using MALDI-TOF Mass Spectrometryen_US
dc.typeArticleen_US

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