Sıçanlarda minosiklinin beyin iskemi-reperfüzyon hasarıüzerine etkilerinin incelenmesi
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Tarih
2013
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Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada ratlarda serebral iskemi-repefüzyona bağlı gelişen hasara minosiklinin etkileri ve etkinliği araştırılmasını amaçladık. Yöntem: Bu çalışma Mustafa Kemal Üniversitesi Tıp Fakültesi Beyin ve Sinir Cerrahisi Anabilim Dalı'nda M.K.Ü. Deneysel Araştırmalar Merkezi'nin (DAM) laboratuar imkanları kullanılarak 200-250 gram 24 adet erkek Wistar Albino sıçan 3 gruba ayrılarak, anterior servikal diseksiyonla bilateral a. carotis communis'ler ortaya konduktan sonra n. vaguslar ekarte edilerek uygulanan oklüzyonunun ardında iskemi-reperfüzyon hasarı oluşturularak gerçekleştirildi. Minosikliniskemi/ reperfüzyon grubuna iskemi öncesi 48. Saat, 24. Saat ve 1. Saatte minosiklin 90 mg./kg. p.o. uygulandı, tanımlanan modele uygun iskemi-reperfüzyon hasarı yapıldı. Reperfüzyon sonrası alınan kan ve beyin dokusunda MDA, SOD, GSH-PX, CAT düzeyleri ve kanda CK, CK-BB, LDH, NSE, protein S100b düzeyleri ölçüldü ve histopatolojik değişiklikler incelendi. Verilerin istatistiksel değerlendirmesi SPSS v15 programı kullanılarak yapıldı. Bulgular: Yapılan histopatolojik değerlendirmelerde ışık mikroskobu görüntülerinde iskemi-reperfüzyon+minosiklin grubunda iskemi-reperfüzyon grubuna göre dejenerasyon bulgularında kısmi düzelme olduğu gözlenmiştir. İstatistiksel analizde, iskemi-reperfüzyon+minosiklin grubunda iskemi-reperfüzyon grubuna göre vakuolizasyonda (p<0,001), konjesyonda (p<0,05), eozinofilik dejenerasyonda (p<0,001) düzeylerinde anlamlı düzelme saptandı. Biyokimyasal değerlerin istatistiksel analizinde iskemi-reperfüzyon+minosiklin grubunda iskemi-reperfüzyon grubuna göre MDA (p<0,001), NO (p<0,05), CK (p<0,05), CK-MB (p<0,05) değerlerinde anlamlı fark saptandı. xiii Sonuçlar: Minosiklinin beyin iskemi-reperfüzyon hasarı üzerinde etkilerinin incelendiği bu çalışmada minosiklin uygulanan grupta kısmi düzelme bulguları saptandı. Minosiklinin beyin iskemi-reperfüzyon hasarında etkilerinin çoklu merkezli çalışmalarla incelenmesi ve standardize edilmesi gerekmektedir.
Objective: It is aimed to assess the effects and effectiveness of minocycline on injury developed by cerebral ischemia-reperfusion in rats in the present study. Material and Methods: This study was performed at Mustafa Kemal University Faculty of Medicine, Department of Brain and Nerve Surgery, using the laboratory facility of Mustafa Kemal University Experimental Research Center, and after dividing 24 male Wistar Albino rats, weighing 200 to 250 grams, into 3 groups, following exhibition of common carotid artery bilaterally by anterior cervical dissection and exclusion of vagus nerve, ischemia-reperfusion injury was performed by occlusion. Minocycline 90 mg/kg p.o. was administered to rats in ischemiareperfusion group at 48th, 24th, and 1st hours pre-ischemia, and ischemia-reperfusion was applied as appropriate to the model described. Following reperfusion MDA, SOD, GSH-PX, CAT levels in blood and brain tissue, and CK, CK-BB, LDH, NSE, protein S100b levels in blood were measured and the histopathological changes were monitored. Data were statistically assessed using SPSS v15. Results: Regarding histopathological evaluation, symptoms of degeneration were partially improved in the ischemia-reperfusion+minocycline group when compared to the ischemia-reperfusion sole group. When analyzed statistically, significant improvement in vacuolization (p<0,001), congestion (p<0,05), and eosinophillic degeneration (p<0,001)levels were found in the ischemia-reperfusion+minocycline group when compared to the ischemia-reperfusion sole group. In the statistical analysis of biochemical parameters, significant differences in MDA (p<0,001), NO xv (p<0,05), CK (p<0,05), CK-MB (p<0,05) levels in the ischemiareperfusion+ minocycline group when compared to the ischemia-reperfusion sole group. Conclusion: Partial improvement was found in minocycline applied group in this study where the effects of minocycline on brain ischemia-reperfusion injury have been studied. The effects of minocycline on brain ischemia-reperfusion injury need to be standardized and studied in multi-center trials. Key words: Brain, ischemia, reperfusion, minocycline, MDA, NSE, protein S100b.
Objective: It is aimed to assess the effects and effectiveness of minocycline on injury developed by cerebral ischemia-reperfusion in rats in the present study. Material and Methods: This study was performed at Mustafa Kemal University Faculty of Medicine, Department of Brain and Nerve Surgery, using the laboratory facility of Mustafa Kemal University Experimental Research Center, and after dividing 24 male Wistar Albino rats, weighing 200 to 250 grams, into 3 groups, following exhibition of common carotid artery bilaterally by anterior cervical dissection and exclusion of vagus nerve, ischemia-reperfusion injury was performed by occlusion. Minocycline 90 mg/kg p.o. was administered to rats in ischemiareperfusion group at 48th, 24th, and 1st hours pre-ischemia, and ischemia-reperfusion was applied as appropriate to the model described. Following reperfusion MDA, SOD, GSH-PX, CAT levels in blood and brain tissue, and CK, CK-BB, LDH, NSE, protein S100b levels in blood were measured and the histopathological changes were monitored. Data were statistically assessed using SPSS v15. Results: Regarding histopathological evaluation, symptoms of degeneration were partially improved in the ischemia-reperfusion+minocycline group when compared to the ischemia-reperfusion sole group. When analyzed statistically, significant improvement in vacuolization (p<0,001), congestion (p<0,05), and eosinophillic degeneration (p<0,001)levels were found in the ischemia-reperfusion+minocycline group when compared to the ischemia-reperfusion sole group. In the statistical analysis of biochemical parameters, significant differences in MDA (p<0,001), NO xv (p<0,05), CK (p<0,05), CK-MB (p<0,05) levels in the ischemiareperfusion+ minocycline group when compared to the ischemia-reperfusion sole group. Conclusion: Partial improvement was found in minocycline applied group in this study where the effects of minocycline on brain ischemia-reperfusion injury have been studied. The effects of minocycline on brain ischemia-reperfusion injury need to be standardized and studied in multi-center trials. Key words: Brain, ischemia, reperfusion, minocycline, MDA, NSE, protein S100b.
Açıklama
Anahtar Kelimeler
Nöroşirürji, Neurosurgery, Beyin, iskemi, reperfüzyon, minosiklin, MDA, NSE, protein S100b.
