Rat ovaryumunda iskemi
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Dosyalar
Tarih
2023
Yazarlar
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Yayıncı
Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Çalışmada ratlarda ovaryum torsiyon/detorsiyon modelinde (iskemi/reperfüzyon), rutin ve taksifolinin olası iyileştirici etkilerinin biyokimyasal ve histolojik analizler ile araştırılması amaçlanmıştır. Çalışmada 70 adet Sprague-Dawley ırkı ratlar; grup 1: kontrol, grup 2: sham, grup 3: torsiyon (T), grup 4: torsiyon/detorsiyon (T/D), grup 5: torsiyon/detorsiyon+dimetilsülfoksit (T/D+DMSO), grup 6: torsiyon/detorsiyon+rutin (T/D+rutin, 80 mg/kg), grup 7: torsiyon/detorsiyon+taksifolin (T/D+Taksifolin, 40 mg/kg) olarak ayrılmıştır. Plazma ve ovaryum örneklerinde biyokimyasal analizler ile ovaryumda histolojik muayeneler gerçekleştirilmiştir. Plazma LPO düzeyleri yönünden T/D+Taksifolin grubunda, kontrol, T ve T/D gruplarına göre azalma olduğu gözlenmiştir (p<0,01). Doku MDA düzeyleri T/D+DMSO, T/D+rutin ve T/D+Taksifolin gruplarında, T/D grubuna göre azalmıştır (p<0,001). Doku Cp düzeyleri T/D+rutin grubunda, T/D+DMSO grubuna kıyasla önemli düzeyde düşük (p<0,01) olarak belirlenmiştir. Doku GSH düzeyleri değerlendirildiğinde; T/D+DMSO grubunda, T/D grubuna göre önemli bir artış olduğu belirlenirken (p<0,05), T/D+rutin ve T/D+taksifolin gruplarında, T/D+DMSO grubuna göre önemli bir azalma gözlenmiştir (p<0,05). Plazma GSH düzeyleri T/D+DMSO grubunda, T/D grubuna göre önemli bir artış göstermiştir (p<0,001). Torsiyon grubunda normal histolojik yapı ile birlikte foliküler yapılanmada bozulmalar ve atretik folikül tespit edilmiştir. Ayrıca intersitisyel alanlar ile corpus luteum ve çevresinde hemoraji ve hiperemi gözlenmiştir. Torsiyon/detorsiyon grubunda, T grubu ile karşılaştırıldığında daha yoğun bir hiperemi ayrıca yaygın bir hemoraji gözlenmiştir. T/D+DMSO grubunun, folikül yapılarındaki bozulmalar ve doku bütünlüğü açısından T/D, T/D+rutin ve T/D+taksifolin gruplarına benzer olduğu görülmüştür. Hemorajik alan yoğunluğunun sham ve T/D+taksifolin grubuna yakın derecede olduğu bununla birlikte hiperemik alanların T, T/D, T/D+rutin ve T/D+taksifolin gruplarına göre azaldığı, hatta sham grubuna yakın olduğu gözlenmiştir. Sonuç olarak T/D+DMSO grubunda, T/D grubuna kıyasla, doku MDA düzeylerinin düşük ve doku ile plazma GSH düzeylerinin yüksek olması, DMSO içinde çözündürülen rutin ve taksifolinin doku GSH düzeylerini T/D+DMSO grubuna göre düşürmesi ile histolojik bulgular birlikte değerlendirildiğinde; DMSO'nun ovaryum torsiyon/detorsiyon hasarında olumlu etkiye sahip bir ajan olabileceği, rutin ve taksifolinin uygulanan dozda ve metodla prooksidan etkinlik göstermiş olabileceği sonucuna varılmıştır.
In this project, it was aimed to investigate the possible amoliorative effects of rutin and taxifolin in the ovarian torsion/detorsion model (ischemia/reperfusion) in rats with biochemical analyzes and histological examinations. Seventy Sprague-Dawley rats, 8-10 weeks old, were used in the study. Rats were divided into; group 1: control, group 2: sham undergoing laparotomy, group 3: torsion (T), group 4: torsion/detorsion (T/D), group 5: torsion/detorsion+dimethylsulfoxide group (T/D+DMSO), group 6: torsion/detorsion +rutin (T/D+rutin, 80 mg/kg), group 7: torsion/detorsion + taxifolin (T/D + taxifolin, 40 mg/kg) . Biochemical parameters were determined in plasma and ovary samples. Histological examinations were performed on ovarian tissues. There was a significant decrease in plasma LPO levels in the T/D+taxifolin group compared to the control, T and T/D groups (p<0.01). When tissue MDA levels are examined; a significant decrease was observed in the T/D+DMSO, T/D+rutin and T/D+taxifolin groups compared to the T/D group (p<0.001). Tissue Cp levels were found to be significantly lower (p<0.01) in the T/D+rutin group compared to the T/D+DMSO group. When tissue GSH levels are evaluated; it was determined that there was a significant increase in the T/D+DMSO group compared to the T/D group (p<0.05), a significant decrease was observed in the T/D+rutin and T/D+taxifolin groups compared to the T/D+DMSO group (p<0.05). When the plasma GSH levels were examined, it was determined that there was a significant increase in the T/D+DMSO group compared to the T/D group (p<0.001). In the torsion group, together with the normal histological structure, deteriorations in follicular structure and atretic follicle were detected. In addition, hemorrhage and hyperemia were observed in interstitial areas, corpus luteum and its surroundings. A more intense hyperemia and extensive hemorrhage were observed in the torsion/detorsion group compared to the T group. It was observed that the T/D+DMSO group was similar to the T/D, T/D+rutin and T/D+taxifolin groups in terms of deterioration in follicle structures and tissue integrity. It was observed that the hemorrhagic area density was close to the sham and T/D+taxifolin groups, however, the hyperemic areas were decreased compared to the T, T/D, T/D+tutin and T/D+taxifolin groups, even close to the sham group. As a result; findings revealed that T/D+DMSO decreased tissue MDA levels and increased tissue and plasma GSH levels compared to T/D group, and that rutin and taxifolin dissolved in DMSO decreased tissue GSH levels compared to T/D+DMSO group. When evaluated together with histological examinations, it was concluded that DMSO may be an agent that has a positive effect on ovarian torsion/detorsion injury, and that rutin and taxifolin may have demonstrated pro-oxidant activity at the applied dose and method.
In this project, it was aimed to investigate the possible amoliorative effects of rutin and taxifolin in the ovarian torsion/detorsion model (ischemia/reperfusion) in rats with biochemical analyzes and histological examinations. Seventy Sprague-Dawley rats, 8-10 weeks old, were used in the study. Rats were divided into; group 1: control, group 2: sham undergoing laparotomy, group 3: torsion (T), group 4: torsion/detorsion (T/D), group 5: torsion/detorsion+dimethylsulfoxide group (T/D+DMSO), group 6: torsion/detorsion +rutin (T/D+rutin, 80 mg/kg), group 7: torsion/detorsion + taxifolin (T/D + taxifolin, 40 mg/kg) . Biochemical parameters were determined in plasma and ovary samples. Histological examinations were performed on ovarian tissues. There was a significant decrease in plasma LPO levels in the T/D+taxifolin group compared to the control, T and T/D groups (p<0.01). When tissue MDA levels are examined; a significant decrease was observed in the T/D+DMSO, T/D+rutin and T/D+taxifolin groups compared to the T/D group (p<0.001). Tissue Cp levels were found to be significantly lower (p<0.01) in the T/D+rutin group compared to the T/D+DMSO group. When tissue GSH levels are evaluated; it was determined that there was a significant increase in the T/D+DMSO group compared to the T/D group (p<0.05), a significant decrease was observed in the T/D+rutin and T/D+taxifolin groups compared to the T/D+DMSO group (p<0.05). When the plasma GSH levels were examined, it was determined that there was a significant increase in the T/D+DMSO group compared to the T/D group (p<0.001). In the torsion group, together with the normal histological structure, deteriorations in follicular structure and atretic follicle were detected. In addition, hemorrhage and hyperemia were observed in interstitial areas, corpus luteum and its surroundings. A more intense hyperemia and extensive hemorrhage were observed in the torsion/detorsion group compared to the T group. It was observed that the T/D+DMSO group was similar to the T/D, T/D+rutin and T/D+taxifolin groups in terms of deterioration in follicle structures and tissue integrity. It was observed that the hemorrhagic area density was close to the sham and T/D+taxifolin groups, however, the hyperemic areas were decreased compared to the T, T/D, T/D+tutin and T/D+taxifolin groups, even close to the sham group. As a result; findings revealed that T/D+DMSO decreased tissue MDA levels and increased tissue and plasma GSH levels compared to T/D group, and that rutin and taxifolin dissolved in DMSO decreased tissue GSH levels compared to T/D+DMSO group. When evaluated together with histological examinations, it was concluded that DMSO may be an agent that has a positive effect on ovarian torsion/detorsion injury, and that rutin and taxifolin may have demonstrated pro-oxidant activity at the applied dose and method.
Açıklama
Anahtar Kelimeler
Biyokimya, Biochemistry, Rutin, Taksifolin, Ovaryum, İskemi/Reperfüzyon, Torsiyon/ Detorsiyon, Rutin, Taxifolin, Ovary, Ischemia/Reperfusion, Torsion/Detorsion.
