Prevalence of methicillin-resistant staphylococci in dogs
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Tarih
2013
Dergi Başlığı
Dergi ISSN
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmanın amacı, köpeklerin nazal mukozalarında metisilin dirençli stafilokokların (MRS) varlığınının ve tür dağılımının belirlenmesidir. Bu amaçla, Hatay’da özel veteriner kliniklerine getirilen 162 köpekten nazal svablar alındı. Metisilin direncinin belirlenmesinde 2 ?g/ml oksasillin içeren mannitollü tuzlu agar kullanıldı. Bakteriyel identifikasyon 16S rRNA dizi analizi ile gerçekleştirildi. Stafilokokal kromozomal kaset tiplendirmesi (SCCmec) için multipleks polimeraz zincir reaksiyonu (mPZR) yapıldı. Antimikrobiyal duyarlılıkları disk diffuzyon yöntemi ile ve antimikrobiyal direnç genleri PZR ile incelendi. Köpeklerin %15.42’ünden (25/162) mecA geni taşıyan MRS izole edildi. Yirmibeş MRS izolatı S. epidermidis (n=12), S. lentus (n=6), S. hominis (n=4), S. warneri (n=1), S. arlettae (n=1) ve S. haemolyticus (n=1) olarak identifiye edildi. S. arlettae’da mecA geni ilk kez belirlendi. Metisilin dirençli S. aureus (MRSA) ve S. pseudintermedius (MRSP) izole edilmedi. SCCmec tip I, II, III ve IV sırasıyla 1, 10, 9 and 5 MRS izolatında belirlendi. Sonuçlar, MRSA dahil MRS suşlarının ortaya çıkışını belirlemek için sürekli surveyansın gerekli olduğunu işaret etmektedir.
The aim of the study was to investigate the occurence and species distribution of methicillin resistant staphylococci (MRS) in the nasal cavity of dogs. Nasal swabs were collected from 162 dogs entering private veterinary clinics in Hatay. Methicillin resistance was detected onto mannitol salt agar containing 2 μg/ml oxacillin and confirmed by mecA Polymerase Chain Reaction (PCR). Bacterial identification was done using 16S rRNA sequencing. Staphylococcal cassette chromosome mec (SCCmec) typing of these isolates were determined by multiplex PCR. Antimicrobial susceptibility testing were performed disk diffusion method and antimicrobial resistance genes were determined by PCR. Methicillin-resistant coagulase negative staphylococci (MRCNS) harbouring mecA were isolated from 15.4% (25/162) of dogs. The species identified were S. epidermidis (n=12), S. lentus (n=6), S. hominis (n=4), S. warneri (n=1), S. arlettae (n=1) and S. haemolyticus (n=1). mecA-mediated methicillin resistance in S. arlettae was described for the first time. Methicillin resistant Staphylococcus aureus (MRSA) and methicillin resistant Staphylococcus pseudintermedius (MRSP) were not detected. SCCmec type I, II, III and IV were identified in 1, 10, 9 and 5 MRS isolates, respectively. The results indicate that continuous surveillance is necessary to determine the emergence of MRS including MRSA.
The aim of the study was to investigate the occurence and species distribution of methicillin resistant staphylococci (MRS) in the nasal cavity of dogs. Nasal swabs were collected from 162 dogs entering private veterinary clinics in Hatay. Methicillin resistance was detected onto mannitol salt agar containing 2 μg/ml oxacillin and confirmed by mecA Polymerase Chain Reaction (PCR). Bacterial identification was done using 16S rRNA sequencing. Staphylococcal cassette chromosome mec (SCCmec) typing of these isolates were determined by multiplex PCR. Antimicrobial susceptibility testing were performed disk diffusion method and antimicrobial resistance genes were determined by PCR. Methicillin-resistant coagulase negative staphylococci (MRCNS) harbouring mecA were isolated from 15.4% (25/162) of dogs. The species identified were S. epidermidis (n=12), S. lentus (n=6), S. hominis (n=4), S. warneri (n=1), S. arlettae (n=1) and S. haemolyticus (n=1). mecA-mediated methicillin resistance in S. arlettae was described for the first time. Methicillin resistant Staphylococcus aureus (MRSA) and methicillin resistant Staphylococcus pseudintermedius (MRSP) were not detected. SCCmec type I, II, III and IV were identified in 1, 10, 9 and 5 MRS isolates, respectively. The results indicate that continuous surveillance is necessary to determine the emergence of MRS including MRSA.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Kafkas Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
19
Sayı
1
