Detection and characterization of phytoplasmas in some cucurbits (Cucurbitaceae) and bindweed (Convolvulaceae) in Hatay Province of Turkey

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dc.contributor.authorÇarpar, Hakan
dc.contributor.authorSertkaya, Gülşen
dc.date.accessioned2024-09-19T16:25:02Z
dc.date.available2024-09-19T16:25:02Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAims: During a disease survey conducted in 2019, typical symptoms including yellowing of the leaves, stunting, shortening of internodes, proliferation of auxiliary shoots (witches'-broom), the bunchy appearance of growth at the ends of stems (rosetting), virescence of flowers or sterility, phyllody, small and deformed leaves and fasciation were observed on suspected cucurbit plants growing in Hatay province. Therefore, this study was carried out to determine the phytoplasma infection(s) on cucurbit crops and bindweed in Hatay province of Turkey.Methods and Results: In total; 4 pumpkins (winter squash: Cucurbita moschata Duchesne), 12 summer squash (Cucurbita pepo L.) and 2 bindweed (Convolvulus arvensis L.) samples were collected from suspected plants in cucurbit fields in Hatay province. Some of the plants (2 pumpkins, 10 summer squash and 2 bindweeds) exhibited symptoms related to phytoplasmas. Total nucleic acid was extracted by CTAB method. PCR amplification of 16S rDNA with phytoplasma specific primer pairs; F1/R0 followed by R16F2n/R2 confirmed the phytoplasma presence. Expected size amplicons of ~1.2 kb were obtained from infected samples from symptomatic cucurbits (12/12) and bindweed (1/2), but not from asymptomatic cucurbit plants (0/4). The sequences of the 4 symptomatic samples were deposited in NCBI GenBank (MT163353, MT163393, MT163396, and MT163469).Conclusions: After sequencing and phylogenetic analyses it was revealed that the squash isolates had 99% sequence identity with “Candidatus phytoplasma trifolii” (16SrVI) and the bindweed isolate had 99% sequence identity with “Candidatus phytoplasma solani” (16SrXII).Significance and Impact of the Study: There is a lack of knowledge on the status of phytoplasma infections and natural hosts in cucurbits in Turkey. According to our knowledge, this is the first report of a member of 16SrVI group, Candidatus phytoplasma trifolii associated with phytoplasma infection in winter squash-pumpkin (C. moschata) and summer squash (C. pepo) in Turkey.en_US
dc.identifier.doi10.37908/mkutbd.1041286
dc.identifier.endpage173en_US
dc.identifier.issn2667-7733
dc.identifier.issue1en_US
dc.identifier.startpage166en_US
dc.identifier.trdizinid1202159en_US
dc.identifier.urihttps://doi.org/10.37908/mkutbd.1041286
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1202159
dc.identifier.urihttps://hdl.handle.net/20.500.12483/16150
dc.identifier.volume27en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofMustafa Kemal Üniversitesi tarım bilimleri dergisi (online)en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCa. phytoplasma solanien_US
dc.subjectCa. phytoplasma trifoliien_US
dc.subjectPhytoplasmaen_US
dc.subjectSummer squash (Cucurbita pepo)en_US
dc.subjectTurkey.en_US
dc.titleDetection and characterization of phytoplasmas in some cucurbits (Cucurbitaceae) and bindweed (Convolvulaceae) in Hatay Province of Turkeyen_US
dc.typeArticleen_US

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