In situ localization of germin gene expression during auxin induced callus formation

Basit Öğe Kaydı
dc.contributor.authorÇalışkan, Mahmut
dc.date.accessioned2019-07-16T15:47:31Z
dc.date.available2019-07-16T15:47:31Z
dc.date.issued2001
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractWheat embryogenic callus was induced by treating immature wheat embryos with 2,4-D. Digoxigenin-labeled germin sense and anti-sense RNA probes were prepared by in vitro transcription. By using these probes it was revealed that there was a striking induction of germin gene expression prior to visible callus formation. Although no germin gene expression was localized on the sections of immature embryos which were not incubated in callus induction medium, following 4 hours incubation in callus induction medium, germin mRNA was localized on the sections of immature embryos. Germin gene expression more conspicuous and widespread on the sections which were incubated for 6 hours in callus induction medium. It was suggested that germin genes induced during callus formation were related to the remodeling of the cell wall to accomplish the transition from precocious germination to callus formation.en_US
dc.description.abstractWheat embryogenic callus was induced by treating immature wheat embryos with 2,4-D. Digoxigenin-labeled germin sense and anti-sense RNA probes were prepared by in vitro transcription. By using these probes it was revealed that there was a striking induction of germin gene expression prior to visible callus formation. Although no germin gene expression was localized on the sections of immature embryos which were not incubated in callus induction medium, following 4 hours incubation in callus induction medium, germin mRNA was localized on the sections of immature embryos. Germin gene expression more conspicuous and widespread on the sections which were incubated for 6 hours in callus induction medium. It was suggested that germin genes induced during callus formation were related to the remodeling of the cell wall to accomplish the transition from precocious germination to callus formation.en_US
dc.identifier.endpage396en_US
dc.identifier.issn1300-0152
dc.identifier.issue4en_US
dc.identifier.startpage387en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/611
dc.identifier.volume25en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryDiğeren_US]
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiyolojien_US
dc.titleIn situ localization of germin gene expression during auxin induced callus formationen_US
dc.title.alternativeOksin'in oluşturduğu kallus dokusunda germin gen ekspresyonunun belirlenmesien_US
dc.typeOtheren_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Caliskan-Mahmut-2001.pdf
Boyut:
122.38 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu