Molecular Characterization of Pseudomonas aeruginosa Isolated From Clinical Bovine Mastitis Cases

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dc.contributor.authorAslantas, Ozkan
dc.contributor.authorTurkyilmaz, Suheyla
dc.contributor.authorKeskin, Oktay
dc.contributor.authorYucetepe, Ayfer Gullu
dc.contributor.authorBuyukaltay, Kaan
dc.date.accessioned2024-09-18T19:54:33Z
dc.date.available2024-09-18T19:54:33Z
dc.date.issued2022
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractThis study aimed to investigate the presence and distribution of virulence determinants and their antimicrobial susceptibilities of 44 Pseudomonas aeruginosa isolates obtained from clinical bovine mastitis cases. In addition, selected 6 P. aeruginosa isolates were further characterized using whole-genome sequencing (WGS). Based on the presence of T3SS-related genes, 25% of the isolates were found to carry exoU and/or exoS genes belonging to invasive (exoU-/exoS+, 18.2%) and cytotoxic (exoU+/exoS-, 6.8%) strains. But, none of the isolates carried both exoU and exoS genes. In terms of other virulence genes examined, various virulence gene profiles were observed among the isolates. The majority of the isolates (72.7%) were susceptible to all tested antimicrobials. Resistance rates to ciprofloxacin and carbapenems (imipenem and meropenem) were determined as 25% and 4.5%, respectively. WGS analysis indicated the presence of different resistome, but fosA genes in all isolates, and different combinations of mutations in gyrA, parC, oprD, efflux pump, and genes playing a role in the regulation of ampC gene expression. Different sequence types (STs) and serotypes were found in representative isolates with the occurrence of the O11-ST235 clone, which is a worldwide multidrug-resistant high-risk clone representing a serious public health threat. The findings of this study provide valuable information on P. aeruginosa isolated from clinical bovine mastitis cases and current antimicrobial resistance levels and virulence determinants.en_US
dc.description.sponsorshipScientific Research Fund of Hatay Mustafa Kemal University [21.GAP.031]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Fund of Hatay Mustafa Kemal University with a grant number of 21.GAP.031.en_US
dc.identifier.doi10.9775/kvfd.2022.28118
dc.identifier.endpage759en_US
dc.identifier.issn1300-6045
dc.identifier.issn1309-2251
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85144293314en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage747en_US
dc.identifier.trdizinid1170617en_US
dc.identifier.urihttps://doi.org/10.9775/kvfd.2022.28118
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1170617
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7794
dc.identifier.volume28en_US
dc.identifier.wosWOS:000898825000001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntimicrobial resistanceen_US
dc.subjectClinical bovine mastitisen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectVirulenceen_US
dc.subjectWhole-genome sequencingen_US
dc.titleMolecular Characterization of Pseudomonas aeruginosa Isolated From Clinical Bovine Mastitis Casesen_US
dc.typeArticleen_US

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