Identification of genotypically mixed Cryptosporidium parvum populations in humans and calves
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Tarih
2003
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Genotypic analyses of Cryptosporidium parvum oocysts have divided the species into two genotypes, referred to as type I and type 2. Although humans are Susceptible to both types, mixed type 1/type 2 infections have rarely been identified. The paucity of mixed infections could be explained by the predominance of one type over the other in mixed infections, or by the poor sensitivity of restriction fragment length polymorphism (RFLP) analyses for detecting subpopulations. Using a type-specific real-time PCR assay capable of detecting type I or type 2 constituting as little as 0.01% of the population, archived and new isolates of human, bovine, and mouse origin were genotyped. Mixed type 1/type 2 infections were identified in humans and calves, including in samples previously found to be homogeneous by RFLP. Isopycnic fractionation of mixed isolates revealed that type I and type 2 oocysts differ in their sedimentation properties. The detection of a type I subpopulation in serially-propagated bovine isolates indicates that type I and type 2 are stably maintained during long-term passage. Together with recently reported experimental bovine and ovine type I infections, the persistence of type I subpopulation in experimentally infected animals Suggests that animals may play a previously unrecognized role in the maintenance of C. parvum type I. (C) 2003 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Cryptosporidium parvum, cryptosporidiosis, subpopulation, mixed infection
Kaynak
Molecular and Biochemical Parasitology
WoS Q Değeri
Q1
Scopus Q Değeri
Q3
Cilt
130
Sayı
1
