Identification of genotypically mixed Cryptosporidium parvum populations in humans and calves

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dc.contributor.authorTanriverdi, S
dc.contributor.authorArslan, MÖ
dc.contributor.authorAkiyoshi, DE
dc.contributor.authorTzipori, S
dc.contributor.authorWidmer, G
dc.date.accessioned2024-09-18T20:13:35Z
dc.date.available2024-09-18T20:13:35Z
dc.date.issued2003
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractGenotypic analyses of Cryptosporidium parvum oocysts have divided the species into two genotypes, referred to as type I and type 2. Although humans are Susceptible to both types, mixed type 1/type 2 infections have rarely been identified. The paucity of mixed infections could be explained by the predominance of one type over the other in mixed infections, or by the poor sensitivity of restriction fragment length polymorphism (RFLP) analyses for detecting subpopulations. Using a type-specific real-time PCR assay capable of detecting type I or type 2 constituting as little as 0.01% of the population, archived and new isolates of human, bovine, and mouse origin were genotyped. Mixed type 1/type 2 infections were identified in humans and calves, including in samples previously found to be homogeneous by RFLP. Isopycnic fractionation of mixed isolates revealed that type I and type 2 oocysts differ in their sedimentation properties. The detection of a type I subpopulation in serially-propagated bovine isolates indicates that type I and type 2 are stably maintained during long-term passage. Together with recently reported experimental bovine and ovine type I infections, the persistence of type I subpopulation in experimentally infected animals Suggests that animals may play a previously unrecognized role in the maintenance of C. parvum type I. (C) 2003 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipNIAID NIH HHS [N01AI25466, AI052781] Funding Source: Medlineen_US
dc.identifier.doi10.1016/S0166-6851(03)00138-5
dc.identifier.endpage22en_US
dc.identifier.issn0166-6851
dc.identifier.issn1872-9428
dc.identifier.issue1en_US
dc.identifier.pmid14550892en_US
dc.identifier.scopus2-s2.0-0141783687en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage13en_US
dc.identifier.urihttps://doi.org/10.1016/S0166-6851(03)00138-5
dc.identifier.urihttps://hdl.handle.net/20.500.12483/9274
dc.identifier.volume130en_US
dc.identifier.wosWOS:000185224900002en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMolecular and Biochemical Parasitologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCryptosporidium parvumen_US
dc.subjectcryptosporidiosisen_US
dc.subjectsubpopulationen_US
dc.subjectmixed infectionen_US
dc.titleIdentification of genotypically mixed Cryptosporidium parvum populations in humans and calvesen_US
dc.typeArticleen_US

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