SYNERGISTIC ACTIVITIES OF TWO PROPOLIS WITH AMPHOTERICIN B AGAINST SOME AZOLE-RESISTANT CANDIDA STRAINS. PART II
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Tarih
2010
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Incdtp-Icpi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Candida strains are opportunistic pathogenic fungi in humans which can cause either septicaemic or mucosal infections. In this study, we aimed to investigate the existance of synergistic activities of two types of propolis (Hatay and Bursa) with Amphotericin B against some azole-resistant Candida strains. To evaluate cytotoxicity of propolis for human cells, the HEp-2 cell line (human larynx epidermoid carcinoma cell line) was used. The cytotoxicity of propolis samples was determined on a conventional haemocytometer using the trypan blue exclusion method. The synergystic activity of Hatay and Bursa propolis samples was assayed against four different azole-resistant Candida isolates (C. albicans, C. tropicalis, C. parapsilosis and C.glabrata). MIC values of Candida spp. were determined as described in the CLSI reference methods. The antifungal activities of a combination of this antifungal agent (Amphotericin B) were assessed by the checkerboard test and time-kill curve study. Bursa and Hatay propolis samples have remarkable antifungal activity against C.albicans, C.glabrata, C.tropicalis and C.parapsilosis. Also, the strong synergistic activity between Amphotericin B and propolis (especially Bursa propolis) was found against the azole resistant candida strains. Propolis may be an important prospect in the treatment of azole-resistant yeast. The effects of these two kinds of propolis against the Candida spp. in vitro are promising.
Açıklama
3rd International Conference on Advanced Materials and Systems -- SEP 16-18, 2010 -- Bucharest, ROMANIA
Anahtar Kelimeler
Propolis, candida strains, Amphotericin B
Kaynak
Proceedings of The 3rd International Conference on Advanced Materials and Systems
WoS Q Değeri
N/A
