Arsenate V induced glutathione efflux from human erythrocytes
Yükleniyor...
Tarih
2012
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: The objective of the present study was to investigate if arsenate V exposure results in glutathione efflux from human erythrocytes. Procedure: The changes in intracellular and extracellular nonprotein sulfhydryl and glutathione levels were determined in arsenate (V) exposed erythrocytes. Presence of any cellular membrane damage was assessed by lactate dehydrogenase activity measurement in the supernatant. Results: When erythrocytes were exposed to 10 mM of arsenate (V) for 4h, the intracellular NPSH level decreased to 0.28 +/- 0025 mu mol/ml erythrocyte. In contrast, extracellular nonprotein thiol level was increased to 0.180 +/- 0.010 mu mol/ml erythrocyte in 4h. Extracellular glutathione levels reached to 0.028 +/- 0.001, 0.052 +/- 0.002, and 0.054 +/- 0.004 mu mol/ml erythrocyte with 1, 5, and 10 mM of arsenate (V), respectively. Utilization of MK571 a multi drug resistance-associated protein 1 inhibitor decreased the rate of glutathione efflux from erythrocytes suggesting a role for this membrane transporter in the process. Conclusion: The results of the present study indicate that erythrocytes efflux glutathione when exposed to arsenate (V). (C) 2011 Elsevier GmbH. All rights reserved.
Açıklama
Anahtar Kelimeler
Arsenate, Glutathione, Human erythrocytes, Membrane transport
Kaynak
Journal of Trace Elements in Medicine and Biology
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
26
Sayı
1
