Arsenate V induced glutathione efflux from human erythrocytes

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dc.contributor.authorYildiz, Deniz
dc.contributor.authorCakir, Yeliz
dc.date.accessioned2024-09-18T20:02:38Z
dc.date.available2024-09-18T20:02:38Z
dc.date.issued2012
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractObjective: The objective of the present study was to investigate if arsenate V exposure results in glutathione efflux from human erythrocytes. Procedure: The changes in intracellular and extracellular nonprotein sulfhydryl and glutathione levels were determined in arsenate (V) exposed erythrocytes. Presence of any cellular membrane damage was assessed by lactate dehydrogenase activity measurement in the supernatant. Results: When erythrocytes were exposed to 10 mM of arsenate (V) for 4h, the intracellular NPSH level decreased to 0.28 +/- 0025 mu mol/ml erythrocyte. In contrast, extracellular nonprotein thiol level was increased to 0.180 +/- 0.010 mu mol/ml erythrocyte in 4h. Extracellular glutathione levels reached to 0.028 +/- 0.001, 0.052 +/- 0.002, and 0.054 +/- 0.004 mu mol/ml erythrocyte with 1, 5, and 10 mM of arsenate (V), respectively. Utilization of MK571 a multi drug resistance-associated protein 1 inhibitor decreased the rate of glutathione efflux from erythrocytes suggesting a role for this membrane transporter in the process. Conclusion: The results of the present study indicate that erythrocytes efflux glutathione when exposed to arsenate (V). (C) 2011 Elsevier GmbH. All rights reserved.en_US
dc.identifier.doi10.1016/j.jtemb.2011.11.003
dc.identifier.endpage58en_US
dc.identifier.issn0946-672X
dc.identifier.issue1en_US
dc.identifier.pmid22177801en_US
dc.identifier.scopus2-s2.0-84857801862en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage53en_US
dc.identifier.urihttps://doi.org/10.1016/j.jtemb.2011.11.003
dc.identifier.urihttps://hdl.handle.net/20.500.12483/7929
dc.identifier.volume26en_US
dc.identifier.wosWOS:000303224700010en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofJournal of Trace Elements in Medicine and Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectArsenateen_US
dc.subjectGlutathioneen_US
dc.subjectHuman erythrocytesen_US
dc.subjectMembrane transporten_US
dc.titleArsenate V induced glutathione efflux from human erythrocytesen_US
dc.typeArticleen_US

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