Localization of germin genes and their products in developing wheat coleoptiles
[ N/A ]
Date
2004
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Springer-Verlag Singapore Pte Ltd
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
Germination is a process which characterized with nescient synthesis of genes. Among the genes synthesized during the germination of wheat embryos, germin genes, proteins and their enzymatic activity were defined. Germin is a water soluble homopentameric glycoprotein which is remarkable resistant to degradation by a broad range of proteases including pepsin. Germin proteins found to have strong oxalate oxidase activity which produces hydrogen peroxide by degrading oxalic acid. The current study, aimed to localize the germin genes, proteins and enzymatic activities in developing coleoptiles which is a rapidly growing protective tissue of leaf primordium and shoot apex. Non-radioactively labeled germin riboprobes were employed to localize germin mRNAs in situ. FITC (Fluorescein isothiocyanate) and alkaline phosphatase linked anti-germin antibodies were used to localize germin proteins under the fluorescence and light microscopy and finally germin enzymatic activity was localized by using appropriate enzyme assay. The results revealed that in coleoptiles germin genes, proteins and their enzymatic activity were predominantly associated with the cells of epidermis and vascular bundle sheath cells.
Description
Keywords
coleoptile development, germin genes, germination, oxalate oxidase
Journal or Series
Journal of Biochemistry and Molecular Biology
WoS Q Value
Q3
Scopus Q Value
N/A
Volume
37
Issue
3
