Localization of germin genes and their products in developing wheat coleoptiles

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dc.contributor.authorCaliskan, M
dc.contributor.authorOzcan, B
dc.contributor.authorTuran, C
dc.contributor.authorCuming, AC
dc.date.accessioned2024-09-18T20:11:34Z
dc.date.available2024-09-18T20:11:34Z
dc.date.issued2004
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractGermination is a process which characterized with nescient synthesis of genes. Among the genes synthesized during the germination of wheat embryos, germin genes, proteins and their enzymatic activity were defined. Germin is a water soluble homopentameric glycoprotein which is remarkable resistant to degradation by a broad range of proteases including pepsin. Germin proteins found to have strong oxalate oxidase activity which produces hydrogen peroxide by degrading oxalic acid. The current study, aimed to localize the germin genes, proteins and enzymatic activities in developing coleoptiles which is a rapidly growing protective tissue of leaf primordium and shoot apex. Non-radioactively labeled germin riboprobes were employed to localize germin mRNAs in situ. FITC (Fluorescein isothiocyanate) and alkaline phosphatase linked anti-germin antibodies were used to localize germin proteins under the fluorescence and light microscopy and finally germin enzymatic activity was localized by using appropriate enzyme assay. The results revealed that in coleoptiles germin genes, proteins and their enzymatic activity were predominantly associated with the cells of epidermis and vascular bundle sheath cells.en_US
dc.identifier.endpage342en_US
dc.identifier.issn1225-8687
dc.identifier.issue3en_US
dc.identifier.pmid15469716en_US
dc.identifier.scopus2-s2.0-3142680775en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage339en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12483/8952
dc.identifier.volume37en_US
dc.identifier.wosWOS:000221742200011en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer-Verlag Singapore Pte Ltden_US
dc.relation.ispartofJournal of Biochemistry and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcoleoptile developmenten_US
dc.subjectgermin genesen_US
dc.subjectgerminationen_US
dc.subjectoxalate oxidaseen_US
dc.titleLocalization of germin genes and their products in developing wheat coleoptilesen_US
dc.typeArticleen_US

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