Investigation of Sensitivity of Rapid Diagnosis Tests in Patients with Suspected Malaria

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dc.authorscopusid6506955731
dc.authorscopusid8927152600
dc.authorscopusid56195646300
dc.authorscopusid57202031650
dc.authorscopusid58927870600
dc.contributor.authorÇulha, Gülnaz
dc.contributor.authorÖnlen, Yusuf
dc.contributor.authorÇabalak, Mehmet
dc.contributor.authorKaya, Tuğba
dc.contributor.authorKüçükeser, Burcu
dc.date.accessioned2024-09-19T15:48:32Z
dc.date.available2024-09-19T15:48:32Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractObjective: Malaria has been eradicated in Türkiye as of 2010, but there are imported cases. In this study, we aimed to compare the diagnostic value of two rapid tests; SD Bioline Malaria Ag Pf/Pan (SD-Pf/Pan) and SD Bioline Malaria Ag Pf/Pv (SD-Pf/Pv) with microscopy and real time-polymerase chain reaction (RT-PCR). Methods: Blood samples were taken from all participants. Thick drop smears were prepared. Thick drop smears were examined for malaria positive/negative distinction under the light microscopy. Then, two rapid diagnostic tests (SD-Pf/Pan and SD-Pf/Pv) were performed. After DNA extraction from blood samples, RT-PCR was typed. The data were evaluated with SPSS 21 program of statistics. Results: A total of 30 cases out of 66 suspected malaria cases were detected as positive with microscopy and RT-PCR. Twenty-seven patients were found positive with both SD-Pf/Pan and SD-Pf/Pv tests. Based on the microscopic results as a reference method, SD-Pf/Pan and SD-Pf/Pv rapid diagnostic tests had a 90% sensitivity, 100% specificity, 100% positive predictive value (PPV), and 92.86% negative predictive value (NPV). Based on the RT-PCR results as a reference method, for detection of P. falciparum, both tests had a 95.65% sensitivity, 100% specificity, 100% PPV, and 88.89% NPV. Moreover, while SD-Pf/Pv had a sensitivity, specificity, PPV, and NPV of 100% in detection of P. vivax; SD-Pf/Pan has a 77.78% sensitivity of, 61.90% specificity of, 46.67% PPV, and 86.67% NPV SD-Pf/Pan for detection of PAN. Conclusion: As a result, high sensitivity and specificity were detected in both kits in the diagnosis of malaria infections caused by P. falciparum and P. vivax. Rapid diagnostic tests can be used safely in diagnosis however the diagnosis should be supported by microscopy and RT-PCR methods when they are applicable. © 2024 Turkish Society for Parasitology-Available online at www.turkiyeparazitolderg.org.en_US
dc.description.sponsorshipHatay Mustafa Kemal Universityen_US
dc.identifier.doi10.4274/tpd.galenos.2024.38358
dc.identifier.endpage7en_US
dc.identifier.issn1300-6320
dc.identifier.issue1en_US
dc.identifier.pmid38449360en_US
dc.identifier.scopus2-s2.0-85187199756en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1en_US
dc.identifier.trdizinid1239923en_US
dc.identifier.urihttps://doi.org/10.4274/tpd.galenos.2024.38358
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1239923
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15136
dc.identifier.volume48en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherGalenos Publishing Houseen_US
dc.relation.ispartofTurkiye Parazitoloji Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectHatayen_US
dc.subjectMalariaen_US
dc.subjectrapid diagnosis testen_US
dc.subjectreal-time polymerase chain reactionen_US
dc.titleInvestigation of Sensitivity of Rapid Diagnosis Tests in Patients with Suspected Malariaen_US
dc.title.alternativeSıtma Şüpheli Hastalarda Hızlı Tanı Kitlerinin Duyarlılığının Araştırılmasıen_US
dc.typeArticleen_US

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