Chemiresistive CuO sensors for label-free C-reactive protein detection
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Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Science Sa
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
In this study, we developed fast and highly sensitive, label-free Ca-doped cupric oxide (CuO) based che-miresistive biosensors to detect C-reactive protein (CRP). The Successive Ionic Layer Adsorption and Reaction (SILAR) method was used to deposit pure and Ca-doped CuO thin films on interdigitated gold electrodes. Film surfaces were functionalized with monoclonal anti-C-reactive protein antibody (anti-CRP) by random physisorption method. Sensor fabrication steps were monitored using the linear sweep vol-tammetry method. Scanning electron microscopy was used to investigate the surface morphology. Energy-dispersive X-ray spectroscopy was used to determine the elemental composition. The structure of the films was investigated by the powder X-ray diffractometry method. Sensor parameters were determined by the change in sensor resistance over time. The sensors showed a good response to CRP with a linear detection range of 0.1-5.0 pg/ml, which was within the clinically significant range. Detection limits of 49.61 and 48.81 ng/ml were estimated using the standard deviation of the y-intercept and the standard deviation of the regression line, respectively. Dynamic response times of all fabricated sensors were found to be less than 30 s (c) 2022 Elsevier B.V. All rights reserved.
Description
Keywords
Chemiresistor, Biosensors, C-reactive protein, Label -free detection, CuO thin film, Linear sweep voltammetry
Journal or Series
Journal of Alloys and Compounds
WoS Q Value
Q1
Scopus Q Value
Q1
Volume
926
