Orak hucre anemili hastalarda fetuin-a, inflamasyon belirtecleri ve antioksidan sistemlerin degerlendirilmesi
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Tarih
2013
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Hatay Mustafa Kemal Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Orak hücre anemisi, Çukurova bölgesinde yaygın görülen eritrositlerde aşırı reaktif oksijen radikali üretimi, vasküler seviyede kronik inflamasyona sebebiyet veren mikrovasküler hipoksik ve reperfüzyon süreci ile karakterize olan konjenital bir hemoglobinopatidir. Bu çalışmada, Orak hücre anemisinde Fetuin-A seviyesi ile bu hastalarda Fetuin-A‟nın inflamasyon ve oksidatif stress ile ilişkisinin gösterilmesi amaçlandı.Yöntem: Çalışmaya Orak hücre anemisi hastası (OHA) olan yetişkin yaş grubu hastalar (n=35) ve sağlıklı bireyler (n=35) dâhil edildi. Tüm örneklerin hematolojik verileri incelendikten sonra hemolizattan süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSH-Px) ve malondialdehit (MDA) analizi yapıldı. Serum örneklerinden Fetuin-A, TNF-Alfa, IL-6, Nitrik Oksit (NO) ve Myeloperoksidaz (MPO) çalışıldı. Bulgular: Kontrol grubu ile OHA grubu karşılaştırıldığında Fetuin-A (p<0,001), TNF-Alfa (p<0,05) ve MDA seviyeleri (p<0,001), OHA grubunda istatistiksel olarak yüksek tespit edilirken NO seviyesi düşük tespit edildi (p<0.01). G6PD (p<0,001), SOD (p<0,01) ve MPO (p<0.05) enzim aktiviteleri de benzer şekilde OHA grubunda kontrole göre yüksek tespit edildi. IL-6, CAT ve GSH-Px sonuçları bakımından gruplar arasında farklılık tespit edilmedi. Sonuçlar: Fetuin-A‟nın Orak hücre anemili hastalarda yüksek bulunması ve hem TNF-Alfa hem de MDA ile pozitif yönlü ilgileşim göstermesi, OHA‟nın patofizyolojisinde Fetuin-A‟nın inflamatuvar süreç ve oksidatif stresle yakın ilişkili olduğunu göstermektedir.
Aim: Sickle cell anemia (SCA), common disease in Çukurova region, is a congenitalhemoglobinopathy characterized by production of excessive amounts of reactive oxygen radicals in red blood cells, and microvascular hypoxia-reperfusion process giving rise to chronic inflammation along vascular structure. In the study, Fetuin-A levels and its relationship with inflammation and oxidative stress in SCA were aimed to evaluate.Material & Methods: The study included patients with SCA (n=35) and healthy individuals (n=35) in adult age. After analyzing hematological data, Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GSH-Px) and Malondialdehyde (MDA) were measured from hemolysates. Fetuin-A, TNF-alpha, IL-6, Nitric Oxide (NO) and Myeloperoxidase (MPO) were measured from serum samples. Results: Fetuin-A (p<0.001), TNF-alpha (p<0.05) and MDA (p<0.001) weremeasured significantly higher in SCA group, but NO was decreased (p<0.01). Similarly, G6PD (p<0.001), SOD (p<0.01) and MPO (p<0.01) enzyme activitieswere determined higher in SCA when compared to the control group. However, there was no statistical significance in terms of IL-6, CAT and GSH-Px. Results: Elevated Fetuin-A in patients with SCA and its positive correlations with both MDA and TNF-alpha levels demonstrated that Fetuin-A had an iportant role for pathophysiology of SCA in terms of relationship with inflammatory processes andoxidative stress. Key words: Sickle Cell Anemia, fetuin-A, inflammation, oxidative stress
Aim: Sickle cell anemia (SCA), common disease in Çukurova region, is a congenitalhemoglobinopathy characterized by production of excessive amounts of reactive oxygen radicals in red blood cells, and microvascular hypoxia-reperfusion process giving rise to chronic inflammation along vascular structure. In the study, Fetuin-A levels and its relationship with inflammation and oxidative stress in SCA were aimed to evaluate.Material & Methods: The study included patients with SCA (n=35) and healthy individuals (n=35) in adult age. After analyzing hematological data, Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GSH-Px) and Malondialdehyde (MDA) were measured from hemolysates. Fetuin-A, TNF-alpha, IL-6, Nitric Oxide (NO) and Myeloperoxidase (MPO) were measured from serum samples. Results: Fetuin-A (p<0.001), TNF-alpha (p<0.05) and MDA (p<0.001) weremeasured significantly higher in SCA group, but NO was decreased (p<0.01). Similarly, G6PD (p<0.001), SOD (p<0.01) and MPO (p<0.01) enzyme activitieswere determined higher in SCA when compared to the control group. However, there was no statistical significance in terms of IL-6, CAT and GSH-Px. Results: Elevated Fetuin-A in patients with SCA and its positive correlations with both MDA and TNF-alpha levels demonstrated that Fetuin-A had an iportant role for pathophysiology of SCA in terms of relationship with inflammatory processes andoxidative stress. Key words: Sickle Cell Anemia, fetuin-A, inflammation, oxidative stress
Açıklama
Anahtar Kelimeler
Biyokimya, Biochemistry, Orak Hücre Anemisi, Fetuin-A, İnflamasyon, Oksidatif Stres
