İn vitro lipopolisakkarit uyarımlı karaciğer yangı modelinde alfa-terpineol'ün etkinliğinin araştırılması

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dc.contributor.advisorKüçükgül, Altuğ
dc.contributor.authorTunçer, Ayşe Pinar
dc.date.accessioned2024-05-27T17:24:44Z
dc.date.available2024-05-27T17:24:44Z
dc.date.issued2020en_US
dc.departmentEnstitüler, Sağlık Bilimleri Enstitüsü, Biyokimya Ana Bilim Dalıen_US
dc.description.abstractIn Vitro Lipopolisakkarit Uyarımlı Karaciğer Yangı Modelinde Alfa-terpineol'ün Etkinliğinin Araştırılması Terpinollerin birçok çalışmada antioksidan özelliklerinden dolayı antikanser, antikonvülsant ve antiülser gibi biyolojik etkileri ortaya konulmuştur. Araştırmanın amacını Ecoli'den izole edilen LPS (lipopolisakkarit)'in karaciğer hücrelerine etkin konsantrasyonda uygulanarak oluşturulan yangı modelinde α-terpineol'ün anti-inflamatuar ve anti-apoptotik gibi biyofonksiyonları araştırılması oluşturdu. Bu amaçla deneme materyali olarak sürekli hücre hatlarından insan orijinli HepG2 hücreleri materyal olarak seçildi. Etken madde olarak seçilen LPS ve α-terpineol, ticari olarak temin edildi ve hücrelere farklı konsantrasyonlarda 24 saat uygulandı. Her iki etken maddenin etkin konsantrasyonları, hücre canlılık testleriyle gerçekleştirildi. Süre sonunda alınan mRNA örneklerinden ise, TNF-α, IL-1β ve IL-10, Kaspaz 3, Bax ve Bcl-2 gen ekspresyon düzeyleri gerçek zamanlı kuantatif polimeraz zincir reaksiyonuyla (qRT-PCR) araştırıldı. Araştırmada elde edilen sonuçlara göre LPS'in farklı konsantrasyonları hücrelere uygulanarak kontrol grubuna göre %11.5 oranında hücre kayıplarına neden olan 50 ng/ml konsantrasyonu modelleme için seçildi. Ancak α-terpineolün LPS ile birlikte kullanıldığında 10 μM konsantrasyonunda hücre kayıplarını % 2.12 oranında önlediği bulundu. Çalışmada gen analizleri değerlendirildiğinde LPS'in TNF-α ve IL-1β gen ekspresyonlarını arttırdığı, α-terpineol uygulamasının ise bu durumu tersine çevirdiği tespit edildi. Yine IL-10 gen ekspresyonu LPS tarafından baskılanırken, α-terpineol tarafından anlamlı düzeyde uyarıldığı da ortaya konuldu. Bununla birlikte çalışmada LPS'in kaspaz 3 ve Bax gen ekspresyonlarını arttırdığı, ancak α-terpineol'ün bu stimülasyonu baskıladığı tespit edildi. Ayrıca buna ilaveten anti-apoptotik Bcl-2 gen ekspresyonları LPS tarafından baskılanırken, α-terpineol tarafından uyarıldığı bulundu. Araştırmadan elde edilen verilerle, monoterpen α-terpineol'ün kısa süreli ve düşük olan proliferatif konsantrasyonunun özellikle patojen kaynaklı karaciğer rahatsızlıklarında alternatif bir tedavi ajanı olabileceği görüldü. Bununla birlikte konunun tam aydınlatıabilmesi için in vivo ve daha ileri düzey araştırmalara ihtiyaç duyulduğu kanısına varıldı.en_US
dc.description.abstractDue to the antioxidant properties of terpineols, biological effects such as anticancer, anticonvulsant and antiulcer have been revealed in many studies. The aim of the research was to investigate the biofunctions of α-terpineol such as anti-inflammatory and antiapoptotic in the inflammatory model created by applying LPS isolated from Ecoli to liver cells at an effective concentration. For this purpose, HepG2 cells of human origin from continuous cell lines were selected as the test material. LPS and α-terpineol, selected as the active ingredient, were commercially available and applied to the cells at different concentrations for 24 hours. Effective concentrations of both active ingredients were carried out by cell viability tests. TNF-α, IL-1β and IL-10, caspase 3, Bax and Bcl-2 gene expression levels were investigated by real-time quantitative polymerase chain reaction (qRT-PCR). According to the results obtained in the study, different concentrations of LPS were applied to the cells and 50 ng / ml concentration that caused 11.5 % cell loss compared to the control group was selected for modeling. However, when used together with LPS, αterpineol was found to prevent cell loss at a concentration of 10 μM at a rate of 2.12%. When gene analysis was evaluated in the study, it was found that LPS increased gene expressions of TNFα and IL-1β, and α-terpineol application reversed this situation. The other IL-10 gene expression was also suppressed by LPS and it was significantly stimulated by α-terpineol. Besides, in the study, it was found that LPS increased caspase 3 and Bax gene expressions, while α-terpineol significantly suppressed this stimulation. In addition,Bcl-2 gene expressions were found to be suppressed by LPS and α-terpineol application stimulated this gene expression. With the data obtained from the study, it was seen that the short-term and low proliferative concentration of monoterpene α-terpineol may be an alternative treatment agent especially in pathogen-induced liver disorders. However, it was concluded that in vivo and more advanced studies are needed to fully illuminate the subject.en_US
dc.identifier.endpage49en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://tez.yok.gov.tr/UlusalTezMerkezi/TezGoster?key=fl0Kw4p1rmMDotyKRdYv1MTr0DyGoiWKZO_VemWhZDiJJBPiFCbAw0ivYrvPxogd
dc.identifier.urihttps://hdl.handle.net/20.500.12483/3847
dc.identifier.yoktezid643468en_US
dc.language.isoturen_US
dc.publisherHatay Mustafa Kemal Üniversitesien_US
dc.relation.publicationcategoryTezen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiyokimyaen_US
dc.subjectBiochemistryen_US
dc.subjectLipopolisakkariten_US
dc.subjectinflamasyonen_US
dc.subjectα-terpineolen_US
dc.subjectapoptoz.en_US
dc.subjectLipopolysaccharideen_US
dc.subjectinflammationen_US
dc.subjectα-terpineolen_US
dc.subjectapoptosis. Investıgatıon of the Effectıveness of Alpha-terpineol in the In Vitro lipopolysaccaride Stimulated Liver Inflammation Model Due to the antioxidant properties of terpineolsen_US
dc.subjectbiological effects such as anticanceren_US
dc.subjectanticonvulsant and antiulcer have been revealed in many studies. The aim of the research was to investigate the biofunctions of α-terpineol such as anti-inflammatory and antiapoptotic in the inflammatory model created by applying LPS isolated from Ecoli to liver cells at an effective concentration. For this purposeen_US
dc.subjectHepG2 cells of human origin from continuous cell lines were selected as the test material. LPS and α-terpineolen_US
dc.subjectselected as the active ingredienten_US
dc.subjectwere commercially available and applied to the cells at different concentrations for 24 hours. Effective concentrations of both active ingredients were carried out by cell viability tests. TNF-αen_US
dc.subjectIL-1β and IL-10en_US
dc.subjectcaspase 3en_US
dc.subjectBax and Bcl-2 gene expression levels were investigated by real-time quantitative polymerase chain reaction (qRT-PCR). According to the results obtained in the studyen_US
dc.subjectdifferent concentrations of LPS were applied to the cells and 50 ng / ml concentration that caused 11.5 % cell loss compared to the control group was selected for modeling. Howeveren_US
dc.subjectwhen used together with LPSen_US
dc.subjectα-terpineol was found to prevent cell loss at a concentration of 10 μM at a rate of 2.12%. When gene analysis was evaluated in the studyen_US
dc.subjectit was found that LPS increased gene expressions of TNFα and IL-1βen_US
dc.subjectand α-terpineol application reversed this situation. The other IL-10 gene expression was also suppressed by LPS and it was significantly stimulated by α-terpineol. Besidesen_US
dc.subjectin the studyen_US
dc.subjectit was found that LPS increased caspase 3 and Bax gene expressionsen_US
dc.subjectwhile α-terpineol significantly suppressed this stimulation. In addition,Bcl-2 gene expressions were found to be suppressed by LPS and α-terpineol application stimulated this gene expression. With the data obtained from the studyen_US
dc.subjectit was seen that the short-term and low proliferative concentration of monoterpene α-terpineol may be an alternative treatment agent especially in pathogen-induced liver disorders. Howeveren_US
dc.subjectit was concluded that in vivo and more advanced studies are needed to fully illuminate the subject. Keywen_US
dc.titleİn vitro lipopolisakkarit uyarımlı karaciğer yangı modelinde alfa-terpineol'ün etkinliğinin araştırılmasıen_US
dc.title.alternativeInvestigation of the effectiveness of alpha-terpineol in the in vitro lipopolysaccaride stimulated liver inflammation modelen_US
dc.typeMaster Thesisen_US

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