Yazar "Alp, Harun" seçeneğine göre listele
Listeleniyor 1 - 20 / 27
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Ameliorating effects of CAPE on oxidative damage caused by pneumoperitoneum in rat lung tissue(E-Century Publishing Corp, 2014) Davarci, Isil; Alp, Harun; Ozgur, Tumay; Karcioglu, Murat; Tuzcu, Kasim; Evliyaoglu, Osman; Motor, SedatWe investigated the biochemical and histopathological effects of caffeic acid phenethyl ester (CAPE) against oxidative stress causing lung injury induced by pneumoperitoneum. Twenty-eight rats were selected at random and seven rats were assigned to each of the following groups. The control group (S) was subjected to a sham operation without pneumoperitoneum. The other groups were subjected to CO2 pneumoperitoneum 15 mmHg for 60 min. The laparoscopy group (L) had no additional drugs administered, the laparoscopy + alcohol (LA) group had 1 ml of 70% ethyl alcohol administered 1 h before the desufflation period, and the laparoscopy + CAPE (LC) group had CAPE administered at 10 mu mol/kg 1 h before the desufflation period. The total oxidative status levels of lung and plasma were significantly increased in the LA group as compared with the LC and S group. When the LC group was compared with the L group, there was a decrease in the level of total oxidant status and increase in the levels of total antioxidant status and paraoxonase in lung tissue. The level of total antioxidative status in the S group was increased compared with the L group in lung tissue and bronchoalveolar lavage fluid. TNF-alpha and IL-6 were found significantly elevated in the L group compared with the LC and S groups in bronchoalveolar lavage fluid. There was a similar increase in plasma levels of IL-6. These results were supported by histopathological examination. CAPE was found to considerably reduce oxidative stress and inflammation induced by pneumoperitoneum.Öğe Apoptotic and Anti-inflammatory Effects of Hypericum Perforatum Extract inHuman Basal Cell Carcinoma TE 354.T Cell Line(2021) Celık, Ebru; Kaplan, Halil Mahir; Şingirik, Ergin; Çelik, Muhammed Salih; Alp, HarunObjectives: Basal cell carcinoma is the most common malignancy in in the Caucasian race and is a serious health problem with its ever-increasing incidence and high cost. Although the treatment is surgical, some elderly patients do not accept surgical intervention. In this case, non-surgical treatments come into prominence. The Hypericum perforatum (HP) / St. John's Wort plant has been used in the treatment of many diseases for centuries among local people. Here, we aimed to aimed to elucidate the effect and molecular mechanism of the HP plant on BCC cells.Methods: Human BCC cell lines TE 354.T were acquired from ATCC®, then were cultured in Dulbecco's Modified Eagle's Medium. BCC cells were seeded at a concentration of 2x106cells in each flask (n=12). After incubation, while six of the flasks were applied with a 0.8% HP extract and 99.2% medium for 48 hours, no application was made to the control group. The effects of HP on mitochondrial mediated intrinsic and extrinsic apoptotic pathway agents, cell cycle G2 / M checkpoint kinases and inflammatory mediators were investigated in BCC cancer cells. The expression and activities of these mediators in both groups were evaluated by ELISA test. Results: It was determined that HP extract treatment increased the expression of apoptotic proteins (AIF, GADD153, GRP78, caspase-3, Bax) and cell cycle G2 / M checkpoint kinase (Wee1), though it reduced antiapoptotic protein (Bcl-2) and inflammatory mediators (iNOS, COX-2, cPLA2, NFkB) in BCC cell lines.Conclusion: HP extract can support routine chemotherapy on BCC cells with its apoptotic, anti-inflammatory and anti-cancer effect, and may be a beacon of hope in reducing resistance to chemotherapeutic drugs in the treatment of BCC.Öğe Caffeic acid phenethyl ester (CAPE) protects against acute urogenital injury following pneumoperitoneum in the rat(Taylor & Francis Ltd, 2014) Rifaioglu, Murat Mehmet; Davarci, Mursel; Nacar, Ahmet; Alp, Harun; Celik, Murat; Sefil, Nebihat Kaplan; Inci, MehmetPurpose: Our aim is to determine the biochemical and histologic changes induced in the kidneys, testis and prostate on possible ischemia and reperfusion (I/R) injury caused by pneumoperitoneum (PNP) in a rat model and to evaluate the ability of protective effects of caffeic acid phenethyl ester (CAPE). Methods: Twenty-eight adult male Wistar albino rats were randomly divided to one of three treatment groups, with seven animals in each group. Sham, laparoscopy (L), and laparoscopy plus CAPE (L + C) group were subjected to 60 min of PNP with 15 mmHg one hour before the desufflation period. Total oxidant status (TOS) and total antioxidant status (TAS) levels were determined in kidney, testis, and prostate. Kidney and testis tissues were removed to obtain a histologic score. Also, Johnsen scoring system was used for testicular tissue analysis. Results: L group had significantly higher TOS and lower TAS levels on kidney and testis compared to the other groups. In prostate biochemical analysis, there was not any difference between groups. No difference was found between groups according to kidney and testis tissues' histologic evaluation. In evaluation of Johnsen scoring, L group showed significant lower score compared to the other two groups. Conclusions: Increased intraabdominal pressure (IAP) had an oxidative effect on kidney and testis but not on prostate in rats. Moreover, it could affect the testicular Johnsen score. All these adverse effects of IAP on both kidney and testis could be prevented by CAPE administration.Öğe Comparison of the chronic effects of ribavirin and caffeic acid phenethyl ester (CAPE) on pancreatic damage and hepatotoxicity(E-Century Publishing Corp, 2014) Motor, Sedat; Alp, Harun; Senol, Serkan; Pinar, Neslihan; Motor, Vicdan Koksaldi; Kaplan, Ibrahim; Alp, AyseThis study was aimed to comparison of the effects of the chronic use of the Ribavirin and caffeic acid phenethyl ester (CAPE) on the pancreatic damage and hepatotoxicity in rats. Methods: The rats were given orally 30 mg/kg/day doses of Ribavirin for 30 days, and intraperitoneally 10 mu mol/kg doses of CAPE. The 37 rats were divided into 4 groups: (I) Control (n=7), (II) Ribavirin (R) (n=10), (III) CAPE (n=10), and (IV) R+CAPE (n=10). Results: Ribavirin and CAPE yielded similar results in terms of Serum, total antioxidant status (TAS), total oxidant status (TOS), amylase, lipase, and insulin compared to the control group. However, while Ribavirin provided similar results with the control group in terms of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzymes, the CAPE group had elevated AST and ALT levels compared to the control group. Histopathologic evaluations revealed that CAPE or Ribavirin had no degenerative effects on both the pancreas and liver tissues. In this way, the biochemical results were confirmed by the histopathologic results. Conclusion: It can be concluded that Ribavirin does not lead to any pancreatic damage and hepatotoxicity, and has more beneficial effects than CAPE on especially liver tissue.Öğe Contractile effects of Eryngium kotschyi Boiss. on rat isolated ileum and detrussor muscle(2014) Baydan, Emine; Kartal, Murat; Yurdakök, Begüm; Aslan Erdem, Sinem; İnce, Sinan; Ekici, Hüsamettin; Alp, HarunBu çalışmada, ülkemizdeki endemik bitkilerden Eryngium kotschyi Boiss.’in toprak altı (EKTA) ve toprak üstü (EKTU) kısımlarının izole sıçan ileum ve idrar kesesi kasında farmakolojik etkinliği araştırıldı. Bitki ekstrelerinin dokulardaki etkinliği tek, agonist (asetilkolin) ve antagonist (atropin, verapamil, oksibutinin-idrar kesesi, papaverin-ileum) varlığında ve kalsiyumsuz ortamda Ca 2+ uygulamaları ile birlikte değerlendirildi. Bitkinin her iki kısmı doku türü, ekstre dozu ve uygulama protokolüne bağlı değişiklik gösterecek şekilde kontraksiyon oluştururken; bu kasılmaların EKTU ve EKTA tek uygulamalarında doza bağımlı, kümülatif uygulamalarında ise dozdan bağımsız olduğu görüldü. Oluşan kasılmaların test edilen antagonistler ile değiştiği; dolayısıyla kontraktil etkinliğin kalsiyum iyonu ve kalsiyum kanallarının uyarılması gibi nonspesifik yolaklara özellikle bağlı olabileceği görüşüne varıldı.Öğe Contractile Effects of Eryngium kotschyi Boiss. on Rat Isolated Ileum and Detrussor Muscle(Kafkas Univ, Veteriner Fakultesi Dergisi, 2014) Baydan, Emine; Kartal, Murat; Yurdakok, Begum; Erdem, Sinem Aslan; Ince, Sinan; Ekici, Husamettin; Alp, HarunThe pharmacological activity of the aerial (EKA) and root (EKR) parts of the endemic plant, Eryngium kotschyi Boiss., on rat isolated ileum and detrusor muscle was investigated. Plant extracts alone and with the presence of agonist (acethylcholine) and antagonist (atropin, verapamile, oxybutinine-detrusor muscle, and papaverine-ileum) drugs, along with Ca2+ applications on calcium-free medium, were applied. Plant extracts induced contraction in ileum and detrusor muscle where the contractions were concentration dependant for EKA and EKR single dose applications in detrusor muscle and concentration-free contractions were observed in cumulative applications for both tissues. Aerial and root parts of Eryngium extracts induced contractions in dose, tissue and protocol dependent manner where the contractions were affected by the tested antagonists, which could be attributed to non-specific pathways including calcium ions and calcium channel stimulations.Öğe Effect of clozapine on locomotor activity and anxiety-related behavior in the neonatal mice administered MK-801(Assoc Basic Medical Sci Federation Bosnia & Herzegovina Sarajevo, 2015) Pinar, Neslihan; Akillioglu, Kubra; Sefil, Fatih; Alp, Harun; Sagir, Mustafa; Acet, AhmetAtypical antipsychotics have been used to treat fear and anxiety disturbance that are highly common in schizophrenic patients. It is suggested that disruptions of N-methyl-d-aspartate (NMDA)-mediated transmission of glutamate may underlie the pathophysiology of schizophrenia. The present study was conducted to analyze the effectiveness of clozapine on the anxiety-related behavior and locomotor function of the adult brain, which had previously undergone NMDA receptor blockade during a developmental period. In order to block the NMDA receptor, male mice were administered 0.25 mg/kg of MK-801 on days 7 to 10 postnatal. In adulthood, they were administered intraperitoneally 0.5 mg/kg of clozapine and tested with open-field and elevated plus maze test, to assess their emotional behavior and locomotor activity. In the group receiving MK-801 in the early developmental period the elevated plus maze test revealed a reduction in the anxiety-related behavior (p<0.05), while the open-field test indicated a decrease in locomotor activity (p<0.01). Despite these reductions, clozapine could not reverse the NMDA receptor blockade. Also, as an atypical antipsychotic agent, clozapine could not reverse impairment in the locomotor activity and anxiety-related behavior, induced by administration of the MK-801 in neonatal period.Öğe The Effects of Caffeic Acid Phenethyl Ester and Ellagic Acid on the Levels of Malondialdehyde, Reduced Glutathione and Nitric Oxide in the Lung, Liver and Kidney Tissues in Acute Diazinon Toxicity in Rats(Medwell Online, 2011) Alp, Harun; Aytekin, Ismail; Atakisi, Onur; Hatipoglu, Namik Kemal; Basarali, Kemal; Ogun, Metin; Buyukbas, SadikThe aim of this study was to investigate the effects of Caffeic Acid Phenethyl Ester (CAPE) and Ellagic Acid (EA) on acitivities of Malondialdehyde (MDA), reduced Glutathione (GSH) and Nitric Oxide (NO) in rat lung, liver and kidney tissues in acute Diazinon (DI) toxicity. Six groups of 6 Sprague Dawley rats were used comprising control, CAPE, EA, DI control, DI+CAPE and DI+EA. Tissue samples were analysed for GSH, MDA and NO levels in lung, liver and kidney tissues. Biochemical parameters were measured colormetrically by spectrophotometer. Control, CAPE and EA groups showed no statistically significant difference whereas DI+medication groups revealed that CAPE and EA increased the level of GSH in liver tissue by blocking the DI effect. NO levels in lung, liver and kidney tissues were significantly increased by DI but CAPE and EA attenuated those levels. In DI+medication groups, MDA levels showed no significant change in kidney and liver tissues but in lung tissues, CAPE and EA reduced the MDA level by blocking the DI effect. It was concluded that CAPE and EA which showed similar effects to each other could be used for protection and support against oxidative stress caused by acute DI intoxication.Öğe THE EFFECTS OF ERDOSTEINE ON COAGULATION IN RATS(Carbone Editore, 2014) Motor, Sedat; Alp, Harun; Yuksel, Rana; Erden, Ersin Sukru; Oktar, Suleyman; Celik, Salih; Cayirci, GokhanBackground and objectives: Erdosteine, a thiol derivative in the same group as N-acetyl cysteine, belongs to a class of medicines called expectorants. Aim: It was aimed to determine effects of erdosteine (ERD) on coagulation. Material and methods: A total of 23 female rats were divided into three groups as control (C) (n=7), ERD-10 (n=7), ERD-50 (n=9). Rats in C group were given dilution of 0.3 ml sodium bicarbonate [NaHCO3]; rats in ERD-10 group were given 10 mg/kg ERD; and rats in ERD-50 group were given 50 mg/kg ERD. The drugs were given for ten days by oral gavage. Blood samples were drawn from the heart 24 hours after the last drug dose, and coagulation parameters were measured with automated analyzers. Results: While Factor VIII and AT-III levels decreased in both ERD-10 and ERD-50 group, Factor IX levels only decreased in ERD-50 group. Also, INR, PT and aPTT were prolonged in the ERD-50 group compared with the control and ERD-10 groups. There was no significant difference between groups for protein C and S. Conclusion: The results showed that erdosteine may increase bleeding tendencies in a dose-related manner. Therefore, we suggest that it should be noted when erdosteine is used together with anticoagulant medications. In addition, patients should be questioned regarding the use of erdosteine before dental or medical operations in order to prevent bleeding abnormalities.Öğe The effects of erdosteine on coagulation in rats(Acta Medica Mediterranea, 2014) Motor, Sedat; Alp, Harun; Yuksel, Rana; Erden, Ersin Sukru; Oktar, Suleyman; Celik, Salih; Cayirci, GokhanBackground and objectives: Erdosteine, a thiol derivative in the same group as N-acetyl cysteine, belongs to a class of medicines called expectorants. Aim: It was aimed to determine effects of erdosteine (ERD) on coagulation. Material and methods: A total of 23 female rats were divided into three groups as control (C) (n=7), ERD-10 (n=7), ERD-50 (n=9). Rats in C group were given dilution of 0.3 ml sodium bicarbonate [NaHCO3]; rats in ERD-10 group were given 10 mg/kg ERD; and rats in ERD-50 group were given 50 mg/kg ERD. The drugs were given for ten days by oral gavage. Blood samples were drawn from the heart 24 hours after the last drug dose, and coagulation parameters were measured with automated analyzers. Results: While Factor VIII and AT-III levels decreased in both ERD-10 and ERD-50 group, Factor IX levels only decreased in ERD-50 group. Also, INR, PT and aPTT were prolonged in the ERD-50 group compared with the control and ERD-10 groups. There was no significant difference between groups for protein C and S. Conclusion: The results showed that erdosteine may increase bleeding tendencies in a dose-related manner. Therefore, we suggest that it should be noted when erdosteine is used together with anticoagulant medications. In addition, patients should be questioned regarding the use of erdosteine before dental or medical operations in order to prevent bleeding abnormalities.Öğe Effects of intralipid and caffeic acid phenethyl ester on neurotoxicity, oxidative stress, and acetylcholinesterase activity in acute chlorpyriphos intoxication(E-Century Publishing Corp, 2014) Ozkan, Umit; Osun, Arif; Basarslan, Kagan; Senol, Serkan; Kaplan, Ibrahim; Alp, HarunChlorpyriphos is one of the most widely used organophosphate (OP) insecticide in agriculture with potential toxicity. Current post-exposure treatments consist of anti-cholinergic drugs and oxime compounds. We studied the effects of intralipid and caffeic acid phenethyl ester (CAPE) on chlorpyriphos toxicity to compose an alternative or supportive treatment for OP poisoning. Methods: Forty-nine rats were randomly divided into seven groups. Chlorpyriphos was administered for toxicity. Intralipid (IL) and CAPE administered immediately after chlorpyriphos. Serum acetylcholinesterase (AChE) level, total oxidant status (TOS), total antioxidant response (TAR), and histologic examination of cerebellum and brain tissue with Hematoxylin-Eosin and immunohistochemical dyes were examined. Results: Serum enzym levels showed that chlorpyriphos and CAPE inhibited AChE while IL alone had no effect, chlorpyriphos and CAPE intensifies the inhibition effect. Significant difference at AChE levels between the chlorpyriphos+IL and chlorpyriphos+CAPE verified that IL has a protective effect on AChE inhibition. TAR levels were significantly increased in all groups except chlorpyriphos group, TOS levels revealed that CAPE and IL decrease the amount of oxidative stress. Histologic examination revealed that neuronal degeneration was slightly decreased at chlorpyriphos+IL group, but CAPE had a significant effect on protection of neuronal degeneration. Conclusion: The results of this study gave us three key points. 1) AChE activity is important for diagnosis of OP intoxication but it has no value for determining the neuro-degeneration. 2) CAPE inhibits AChE activity and may increase the muscarinic-nicotinic hyperactivation. Therefore it should not be used for treatment of OP intoxication. 3) IL decreases the severity of neurodegeneration and symptoms of OP intoxication and it can be used as a supportive agent.Öğe Effects of intralipid and caffeic acid phenyl esther (CAPE) against hepatotoxicity and nephrotoxicity caused by glyphosate isopropylamine (GI)(Sage Publications Inc, 2016) Alp, Harun; Pinar, Neslihan; Dokuyucu, Recep; Kaplan, Ibrahim; Sahan, Mustafa; Senol, Serkan; Karakus, AliThis study was aimed to investigate the protective effects of caffeic acid phenyl esther (CAPE) and Intralipid (IL) against hepatotoxicity and nephrotoxicity caused by acute intoxication of glyphosate (N-phosphonomethyl) glycine) (GI) in rats. Forty-nine Wistar Albino rats were randomly divided into seven groups as: I, Control; II, Intralipid (IL) (18.6 mL/kg, orally); III, CAPE (10 mu mol/kg, intraperitoneally); IV, GI (4 mg/kg/day, intraperitoneally); V, GI + IL; VI, GI+CAPE; and VII, GI + IL + CAPE. Total antioxidant status (TAS) and total oxidant status (TOS) levels were measured in serum samples. Tissues were analyzed with hematoxylin and eosin (H&E) staining protocol. Bcl-2, Bax, and caspase-3 were evaluated by immunohistochemical method. The results revealed that, in hepatic tissues, the TAS levels were lower and the TOS levels were higher in the GI group compared to other groups. In renal tissues, the TAS levels were significantly lower in the GI group than in the control, IL, CAPE, and GI + IL + CAPE groups. The TOS levels were significantly higher in the GI group than in the control group. Moreover, histopathological analysis revealed severe hepatotoxicity in the GI group. In the GI + CAPE + IL group, hepatotoxicity recovered significantly. Nephrotoxicity was also observed in the GI group and moderately reduced in the GI + CAPE group. Biochemical results were confirmed by histopathologic examination. The results also revealed that CAPE and IL, due to their antioxidant effects, have a decreasing effect against both hepatotoxicity and nephrotoxicity caused by GI. Therefore, CAPE and IL may function as potential agents for supportive therapy since they decrease organ damage, or may facilitate the therapeutic effects of the routine treatment of patients with GI poisoning.Öğe Effects of Malathion in fetal kidney tissues in pregnant rats: Teratogenic effects induced by different doses(Veteriner Fakultesi Dergisi, 2012) Alp, Harun; Sak, Muhammet Erdal; Evsen, Mehmet Siddik; Firat, Ugur; Evliyaoglu, Osman; Penbegul, Necmettin; Sancaktutar, Ahmet AliThe aim of this study was to investigate the teratogenic effects of Malathion (ML) induced by different doses on fetal kidney tissues in pregnant rats. A total of 28 Sprague-Dawley pregnant rats were randomly divided into 4 groups of 7 rats each. Depending on ML dose, four groups were formed, including (I) control, (II) ML 2.5 (ML 2.5 mg/kg/day, orally), (III) ML 5 (5 mg/kg/day, orally), and (IV) ML 10 (10 mg/kg/day, orally). ML application started when the male and female were put together (when mating started). Daily ML application was continued until birth. It was determined that in parallel with dose of ML, ML resulted in toxic effects on serum enzymes (acetyl-cholinesterase (AChE), amylase and lipase) and kidney tissues of pregnant rats, and also -regardless of ML dose in fetal kidneys- it led to teratogenic effects in all the doses. Biochemical data wasconfirmed by histopathologic data. We concluded that ML leads to kidney damage in both pregnant and fetal rats as a result of its teratogenic and toxic effects.Öğe Effects of Sildenafil Citrate, Isoniazid, and Streptomycin on Testicular Tissue and Epididymal Semen Quality in Rats(Elsevier Science Inc, 2012) Alp, Harun; Cirit, Umut; Tas, Muzaffer; Rifaioglu, Murat Mehmet; Hatipoglu, Namik Kemal; Aytekin, Ismail; Yucel, MehmetOBJECTIVE To evaluate the effects of isoniazid (INH) and streptomycin (STR) on epididymal semen quality and testicular tissue, and to evaluate the protective effect of sildenafil citrate (SC) on possible testicular toxicity induced by STR and INH in rats. METHODS Eighty adult male Sprague-Dawley rats were divided randomly into 8 groups including control, SC, INH, STR, STR + INH, SC + INH, SC + STR, and SC + INH + STR. After 45 days of treatment, the reproductive organ weights, epididymal semen quality, testicular histopathological findings, levels of serum nitric oxide, testosterone, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were investigated. RESULTS SC significantly increased the epididymal sperm motility and concentration, and the levels of FSH, LH, and testosterone. The STR group had a significantly higher percentage of sperm head defect than the control group (P < .05). The INH group had lower Johnsen Testicular Biopsy Score than the control group (P < .001). Although SC and INH treatment alone did not affect the epididymal semen quality negatively, the SC + INH group had significantly higher spermatozoon tail and total morphologic defect ratios than the control group (P < .05). CONCLUSION It has been concluded from this study that (1) SC has positive effects on spermatogenesis, sperm production, and semen quality; (2) STR affected the testicular biopsy score and spermatozoon head morphology negatively, but positively affected the other spermatologic traits; (3) INH did not effect the epididymal semen quality negatively, but decreased testicular biopsy score; and (4) SC can prevent the spermatozoon head defects induced by STR and can decrease the testicular toxicity induced by INH. UROLOGY 80: 953.e9-953.e14, 2012. (C) 2012 Elsevier Inc.Öğe Effects of Silybum marianum Extract on High-Fat Diet Induced Metabolic Disorders in Rats(Inst Animal Reproduction & Food Research Polish Acad Sciences Olsztyn, 2016) Sayin, Fatma Kubra; Buyukbas, Sadik; Basarali, M. Kemal; Alp, Harun; Toy, Hatice; Ugurcu, VeliSilybum marianum extract (SME) has been used for centuries as a natural remedy for diseases of liver and biliary tract. Lately, it has been promoted as a nutritional supplement for beneficial effects on some risk factors of diabetes and hyperlipidemia. In this study we aimed to determine the effects of SME on high-fat diet (HFD) induced metabolic disorders. Male Sprague Dawley rats were fed HFD for 11 weeks to induce obesity. SME was given to animals for two different durations, for 11 weeks or for 7 weeks. The results showed significant increase in plasma transaminases, total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C), leptin, high sensitive C-reactive protein (hsCRP), glucose and insulin along with significant increase in body mass index (BMI) and liver weights in rats fed the HFD diet compared to rats fed with standard rat diet. SME supplementation for different durations raised improvement in the HFD-induced metabolic disorders such as insulin resistance, hyperlipidemia and hepatopathy at different degrees. Our study concludes that SME can be well considered as an effective supplement to improve insulin and leptin sensitivity and hyperlipidemia and to suppress body weight gain.Öğe Hatay ilinde üniversite hastanesi endokrin polikliniğine başvuran diyabet hastalarının bitkisel ürün kullanımı(2017) Topaloğlu, Meyli; Özsan, Müge; Özer, Cahit; Alp, Harun; Pınar, NeslihanAmaç: Günümüzde insanların tamamlayıcı ve alternatif tıp (TAT) uygulamalarına ilgileri giderek artmaktadır. Bunlar arasında hastalar tarafından en çok tercih edilen bitkisel ürünlerdir. Bitkisel ürünler diyabet hastalarında da oldukça yaygın kullanılmaktadır. Diyabet hastalarında bitkisel ürün kullanımı morbidite ve mortaliteye yol açabilmektedir. Bu çalışma diyabet hastalarında kullanılan bitkisel ürünleri, nasıl kullanıldıkları ve yan etkilerini incelemek üzere planlanmıştır. Gereç ve Yöntem: Kesitsel tanımlayıcı nitelikteki bu çalışma, Mayıs 2016Haziran 2016 tarihleri arasında Mustafa Kemal Üniversitesi Tıp Fakültesi Endokrinoloji polikliniğine başvuran18 yaş üstü görüşmeyi kabul eden 150 hastada yüz yüze görüşme yöntemi ile yapılmıştır. Bulgular: Çalışmamıza katılan diyabet hastalarının % 60,7'si erkek, % 39,3'ü kadın bulunmuştur. Çalışmamıza katılan diyabet hastalarının % 22'si bitkisel ürün kullandığını söylemişlerdir. Bitkisel ürün kullananların % 5,3'ü tarçın, % 4,7'si limon, % 3,3'ü nar ekşisi, % 2,7'si yeşil çay, % 2'si badem, % 1,3'ü civan perçemi, adaçayı, zeytin yaprağı çayı kullandıklarını söylemişlerdir. Bitkisel ürün kullananların hepsi bitkisel ürünü doktora danışmadan kullandığını söylemişlerdir. Kullananların hiçbirisinde yan etki görülmemiştir. Sonuç: Diyabet hastalarının önemli bir kısmı bitkisel ürün kullanmaktadır. Katılımcıların hepsi bitkisel ürünleri doktora danışmadan kullandıklarını söylemektedirler. Hastaların doktora danışmadan bitkisel ürün kullanmaları istenmeyen sonuçlara yol açabilir.Öğe Herbal supplement usage in cardiac patients(Turkish Soc Cardiology, 2017) Pinar, Neslihan; Topaloglu, Meyli; Ozer, Cahit; Alp, HarunObjective: Complementary and alternative medicine (CAM) has become more and more widespread around the world. CAM is a broad term that refers to all medical healthcare services, methods, and practices that are not part of standard medical care, as well as the accompanying theories and beliefs. The aim of the present study was to investigate the use of herbal medicinal products in cardiac patients, as well as the methods of administration of the products. Methods: This descriptive cross-sectional study included 199 patients aged over 18 years who were hospitalized in the Defne Hospital department of cardiology and volunteered to participate in a 20-item survey between April 2016 and June 2016. Results: The study results indicated that 28.6% (n=57) of the patients were using herbal medicinal products and 71.6% (n=142) said they did not. Only 14.03% (n=8) of those who used herbal medicinal products said they used them in consultation with their physician; 85.9% (n=49) had used herbal medicine without consulting their doctor. Of the participants with hypertension, 35.7% of them reported using herbal medicinal products. Of these, 22.5% of them were consuming lemon, 17.5% pomegranate syrup, and 17.5% green tea. Of the participants with cardio-vascular diseases, 23.5% of them stated that they were taking herbal medicinal products. Of these, 25% were consuming green tea, 25% ginger, and 18.8% sage. Conclusion: Herbal medicinal supplements were used by a large portion of the cardiac patients in this study. Furthermore, most of the patients stated that they were using these products without informing their physician, a practice that can have unwanted consequences.Öğe Investigating of Effects of Metformin on Melanoma Skin Cancer(2021) Alp, Harun; Tutun, Hidayet; Altıntaş, Levent; Kaplan, Halil Mahir; Şingirik, Ergin; Kahraman, İbrahimAim: Apoptotic pathways are important for resistance to chemotherapeutics drugs and cancer development. Metformin is a safe, inexpensive and effective drugused to treat type 2 diabetes. Furthermore, although there is evidence of anticancer effects, the mechanisms by which metformin exerts the effects are unclear.Therefore, it was aimed to investigate Bcl-2, kaspaz-3, bax, wee-1, AIF, grp78 and gadd153 levels in metformin treated skin cancer cells. Materials and Methods: The skin cancer cells (B16F10) were treated with metformin and kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF levels were analyzedby using ELISA. Result: Treatment of metformin increased of proapoptotic proteins (kaspase-3, bcl-2, wee 1, gadd153, grp78 and AIF) and decreased antiapoptotic protein (bcl 2). Conclusion: In conclusion, this study showed that metformin has apoptotic effect on melanoma skin cancer.Öğe Investigation of apoptotic effects of hypericum perforatum extract on breast cancer cell line(Harran Üniversitesi, 2019) Alp, Harun; Tutun, Hidayet; Kaplan, Halil Mahir; Şıngırık, Ergin; Altıntaş, LeventAbstract: Hypericum perforatum has biological active contents affecting a variety of proteins such as caspase-3, bcl-2, and bax, which mediate apoptosis known as programmed cell death and exerting anti-inflammatory effect. Apoptotic pathways are important for cancers, chemotherapeutic resistance, and cancer development. Anti-inflammatory agents are also a potential target for cancer. Therefore, it was aimed to investigate the activity of caspase-3 and the expressions of bcl-2, bax, wee 1, gadd153, grp78, AIF, iNOS, COX-2, cPLA2, and NF-κB in H. perforatum extract-treated breast cancer (BC) cells in this study. The activity of caspase-3 and the expressions of these proteins were determined in the cells by ELISA. The HP extract increased the activity of caspase-3 and the expressions of bax, wee 1, gadd153, grp78 and AIF, and decreased the expressions of bcl-2, COX-2, iNOS, cPLA2 and NF-κB in the BC cells. In the light of these findings, HP extract could help to inhibit grow of BC cells and its anti-inflammatory effect may contribute this effect.Öğe Kardiyoloji hastalarında bitkisel ürün kullanımı(2017) Pınar, Neslihan; Topaloğlu, Meyli; Özer, Cahit; Alp, HarunAmaç: Tamamlayıcı ve alternatif tıp (TAT) uygulamaları günümüzde giderek yaygınlaşmaya başlamıştır. TAT geleneksel tıp uygulamaları dışında kalan bütün sağlık hizmetlerini, yöntemlerini, uygulamalarını ve bunlara eşlik eden kuram ve inançları kapsayan geniş bir sağlık alanıdır. Bu çalışma bir kardiyoloji kliniğine yatan kalp hastalarında kullanılan bitkisel ürünleri, nasıl kullanıldıklarını incelemek üzere planlanmıştır. Yöntemler: Kesitsel-tanımlayıcı nitelikteki bu çalışma, Nisan 2016-Haziran 2016 tarihleri arasında Özel Defne Hastanesi Kardiyoloji Servisi'nde yatan 18 yaş üstünde olup görüşmeyi kabul eden 300 hastada 20 sorudan oluşan anket yüz yüze görüşme yöntemi ile yapılmıştır. Bulgular: Çalışmamıza katılan hastaların %28.6'sı (n=57) bitkisel ürün kullandıklarını, %71.6'sı (n=142) bitkisel ürün kullanmadıklarını söylemiştir. Bitkisel ürün kullananların sadece %14.03'ü (n=8) ürünü hekimine danışarak kullandığını söylerken, %85.9'u (n=49) hekimine danışmadan kullandığını söylemişlerdir. Çalışmaya katılan hipertansiyonlu hastaların %35.7'si bitkisel ürün kullanmaktadır. En sık kullanılan bitkisel ürünler %22.5 limon, %17.5 nar ekşisi ve %17.5 yeşil çay idi. Çalışmaya katılan kalp damar hastalarının %23.5'i bitkisel ürün kullandığını beyan etmişlerdir. Bitkisel ürün kullananların %25'i yeşil çay, %25'i zencefil, %18.8'i ada çayı kullandıklarını söylemişlerdir. Sonuç: Kardiyoloji hastalarının önemli bir kısmı bitkisel ürün kullanmaktadır. Büyük bir kısmı sorumlu hekimlerine danışmadan kullandıklarını belirtmektedirler. Hastaların hekimlerine danışmadan bitkisel ürün kullanmaları istenmeyen sonuçlara yol açabilir
