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Öğe Anti-proliferative Effects of Trigonella foeniculum and Eucalyptus camaldulensis on Human Melanoma Cells(Wiley, 2017) Duran, Nizami; Duran, Gulay Gulbol; Kaya, D. Alpaslan; Ay, Emrah; Tumer, Cemil; Demir, Enver Ahmet[Abstract Not Available]Öğe Dose-dependent effects of simvastatin, atorvastatin and rosuvastatin on apoptosis and inflammation pathways on cancerous lung cells(Ankara Univ, 2023) Dikmen, Nursel; Ozkan, Huseyin; Cimen Acikgul, Funda; Camdeviren, Baran; Ay, Emrah; Ambarcioglu, Pinar; Duran, NizamiThe aim of study was to investigate the anti-proliferative and inflammatory effects of atorvastatin, rosuvastatin, and simvastatin in lung cancer. The effects of statins were investigated in Vero, BEAS-2B, and A549 cell lines. In addition to expressions of BAX, BCL-2, TNF alpha, IL-10, IL-6, protein levels of TNF alpha, IL-10, IL-6 were determined. Cell viability and MDA were also measured. While the cell numbers in groups with low doses of statins were found to be approximately 1x106/mL, proliferation was inhibited at higher rates containing high doses. Simvastatin, rosuvastatin, and high dose atorvastatin upregulated the BAX, while high dose of atorvastatin and both doses of rosuvastatin caused downregulation in BCL-2. All statin groups had higher MDA. Simvastatin and high dose rosuvastatin upregulated TNF alpha. While low dose simvastatin and atorvastatin and high dose atorvastatin and rosuvastatin upregulated IL-10, IL6 was upregulated with a low dose of rosuvastatin. TNF alpha was higher in simvastatin and rosuvastatin groups. IL-10 was highest in rosuvastatin groups. Atorvastatin groups had lower IL-6. Although cell numbers have been reduced by all statins, rosuvastatin is more effective on studied genes.Öğe The Effects of Essential Oils of Nigella sativa L. on p53 Gene Expression and Cell Apoptosis in HT-29 Colon Carcinoma Cells(Wiley, 2017) Duran, Nizami; Duran, Gulay Gulbol; Kaya, D. Alpaslan; Ay, Emrah; Demir, Enver Ahmet; Dogan, Hatice[Abstract Not Available]Öğe Effects of nigella sativa l.’s essential oils on multi drug resistant escherichia coli isolates(Bulgarian Society for Microbiology (Union of Scientists in Bulgaria), 2018) Duran, Nizami; Ay, Emrah; Bayraktar, Suphi; Çolak, Sezin; Kaya, Durmuş AlpaslanEscherichia coli is one of the most important human pathogens and is a major cause of nosocomial infections. In this study, it was aimed to investigate the antimicrobial efficacy of Nigella sativa L. against multi drug-resistant E. coli isolates. This study was carried out on multi drug resistant E. coli isolates. In this study, seeds of Nigella sativa L. obtained from spice market were used as material.Multidrug resistant E. coli strains were identified by conventional methods. The antibacterial activity of Nigella sativa L.’s essential oils was performed by disk diffusion test. In addition, when needed, automated systems were used for rapid identification of microorganisms and susceptibility testing identification. The ESBL production of these strains was confirmed by double disc synergy test.In ESBL producing E.coli strains, the MIC range of ampicillin was 4-32 ?g/ml and the MIC range of cefuroxime was found to be 2-16 ?g/ ml. MIC values of ampicillin were range from 0.25 to 4 ?g/ml and MIC of levofloxacin range were 1 to 8 ?g/ml and MIC of cefuroxime range were found to be 0.25-4 ?g/ml. Following treatment with essential oils of Nigella sativa, resistance rates of GSBL producing E.coli strains against all these three antibiotics (ampicillin, levofloxacin and cefuroxime) have been found to decrease statistically significantly (p<0.05). Nigella sativa L.’s essential oils showed complete zone of inhibition of the standart E.coli strains (p<0.05). Besides this, in experiments with multi drug resistant E.coli isolates, it was found that the inhibition zones of Nigella-treated GSBL producing E.coli strains differed from non-treated strains. There was a statistically significant increasing in inhibition zones in drug E.coli isolates (p<0.01).The essential oils of Nigella sativa L. may contain promising antimicrobial components in the treatment of multi drug-resistant Ecoli isolates. This is very important for the discovery of new antimicrobial agents. More extensive clinical trials should be undertaken in this regard. © 2018, Bulgarian Society for Microbiology (Union of Scientists in Bulgaria). All rights reserved.Öğe The frequency of shiga-like toxin (stx1 and stx2) and EHEC-hlyA in food by multiplex PCR(Univ Press, 2017) Onlen, Cansu; Duran, Nizami; Bayraktar, Suphi; Ay, Emrah; Ozer, BurcinAim: The aim of the present study was to determine the frequency of shiga-like toxin (stx1 and stx2) and drug resistance profiles food-borne Escherichia coli O157: H7 in Hatay province, Turkey. Methods: The presence of the virulence genes (stx1, stx2, hlyA) in a total of 150 E. coli isolates were studied with multiplex PCR. Results: A total of 327 salad samples were analyzed. E. coli O157: H7 was detected in 150 (45.8 %) out of 327 analyzed samples. Of these 150 isolates, the presence of hly-A gene was detected in 32 (21.3%) E. coli isolates. A total of five (15.6%) isolates in this 32 hlyA positive isolates had stx2 gene, two (6.3%) of them had stx1 gene and one (3.1%) of the isolates was found to be positive for both stx1 and stx2 genes. It was found that all E. coli O157: H7 isolates were resistant to erythromycin. While the highest rate of antibiotic resistance was observed for ampicillin (68.8%), no antibiotic resistance against cefuroxime, ciprofloxacin and cephaperasone was identified. Conclusions: The results obtained in our province showed that E. coli strains isolated from salad samples were found to have some important virulence genes such as stx1, stx2, and hlyA. The stx2 frequency was found to be higher than stx1 frequency. Also, it was observed that there was not any significant correlation between drug resistance profiles and presence of toxin genes in E. coli O157: H7 strains. As a result, increasing frequency of STEC O157 serotype among foodborne pathogens is a growing public health problem.Öğe Genotyping, drug resistance and virulence factors of Candida species isolated from patients using long-term inhaled steroids(Wiley, 2021) Dikmen, Nursel; Duran, Nizami; Ay, Emrah; Cimen, Funda; Tek, ErhanAims In this study, it was aimed to determine the isolation frequency and species distribution of Candida species isolated from asthmatic patients using long-term inhaled steroids. It was also aimed to determine the drug resistance patterns and the frequency of erg11, HWP1, ALS1, INT1, SAP1 PLB1 genes in isolates. Methods Genotyping of Candida strains isolated from patients and healthy control group was performed by PCR-RFLP method. Drug resistance was investigated phenotypically, and the presence of erg11 resistance genes and HWP1, ALS1, INT1, SAP1 PLB1 virulence genes were investigated by PCR method. Results C albicans was the most isolated species in steroid-using patients and healthy control groups (patients: 44.2%; control group: 30.8%). C tropicalis and C glabrata were found to have the highest rates of non-albicans Candida in patients with 17.4% and 13.77%, respectively. Azole resistance was found to be significantly higher in isolates isolated from patients compared to the control group. Similarly, the presence of erg11 resistance gene was highest in C albicans (17.65%), C glabrata (12.5%) and C tropicalis (8.3%) strains in the control group, while C parapsilosis was highest in patients. (57.1%) and C glabrata (54.2%) strains. Compared to the control group, the virulence of Candida strains isolated from the patients was found to be higher. Presence of HWP1, ALS1, INT1, SAP1 and PLB1 genes in patients were determined as 72.1%, 63.9%, 68.9%, 57.38% and 54.5%, respectively. These rates were 29.4%, 35.3%, 25.5%, 17.7% and 23.5% in the healthy control group, respectively. Conclusions In asthma patients using long-term inhaled steroids, both Isolation rates of Candida species, drug resistance rates, presence of virulence genes were found to be significantly higher in patients than in the control group. We think that this may be due to the suppression of cellular immunity by long-term steroid use.Öğe Hatay bölgesinde bazı tıbbi bitkilerin çeşitli kanser hücre hatları üzerinde antikanserojen aktivitelerinin araştırılması(Hatay Mustafa Kemal Üniversitesi, 2019) Ay, Emrah; Duran, Ni̇zami̇Amaç: Çalışmamızda bölgemiz için endemik olan, gerek doğal ve gerekse de kültive edilerek tarımı yapılan Hypericum perforatum, Thymbra spicata, Myrtus communis, Laurus nobilis, Rosmarinus officinalis ve Lavandula angustifolia bitkilerinin esansiyel yağlarının insan kolon kanseri (HCT-116), akciğer kanseri (A-549) ve malign melanom (A-2058) hücre hatlarındaki antikanserojen etkilerinin varlığını araştırmayı amaçladık. Materyal ve Yöntem: Bitkilerin kimyasal komponent analizleri GC-MS yöntemi ile belirlendi. Çalışmada bitkisel esansiyel yağların non-toksik konsantrasyonları sağlıklı hücre hattı olarak seçilen Vero hücreleri üzerinde belirlenmiştir. Antiproliferatif etkinlik çalışmaları Vero hücreleri üzerinde tespit edilen non-toksik konsantrasyonlar dahilinde gerçekleştirildi. Kanser hücre hatları olarak kolorektal kanser hücre hattı (HCT-116), insan melanoma hücre hattı (A-2058) ve akciğer karsinomu hücre hattı (A-549) kullanıldı. Aktivite testleri 96 saat süreyle (24., 48., 72., 96.saatlerde) değerlendirmeler yapılarak gerçekleştirildi. Sitotoksisite testleri için MTT yöntemi kullanıldı. Bulgular: Thymbra spicata, Laurus nobilis, Rosmarinus officinalis, Hypericum perforatum, Lavandula angustifolia ve Myrtus communis esansiyel yağlarının GC-MS analizi sonucunda en yüksek oranda tespit edilen bileşenlerinin sırasıyla; Carvacrol (%58.32), Eucalyptol (%57.42), Eucalyptol (%25.29), α-Pinene (%38.24), L-Fenchone (%45.86) ve Eucalyptol (%36.51) olarak tespit edilmiştir. Çalışmada en güçlü antiproliferatif etkinliği Thymbra spicata esansiyel yağlarının A-2058 melanoma hücreleri üzerinde 0.0195 g/ml'lik konsantrasyonda gösterdiği tespit edilirken, en düşük antiproliferatif etkinliğin ise Hypericum perforatum esansiyel yağlarının A-2058 hücrelerine karşı 0.312 g/ml konsantrasyonda elde edildiği tespit edilmiştir. Sonuçlar: Bu sonuçlar mortalite ve morbidite açısından dünyada en önemli hastalıklardan olan kanseree karşı bu esansiyel yağ komponentlerinin oldukça dikkate değer nitelikte antiproliferasyon sağladığı tespit edilmiş olup, yeni ilaç araştırmaları için umut verici bulunmuştur. Çalışma bulgularımızın in-vivo olarak ileri çalışmalarla desteklenmesi gerektiğini oldukça önemli olacaktır.Öğe In vitro cytotoxic activity of Nigella sativa L. On human malignant melanoma cell lines(Certex, INCDTP-ICPI, 2016) Duran, Nizami; Duran, Gulay Gulbol; Ay, Emrah; Kaya, Durmus¸ Alpaslan; Kaya, Madalina Georgiana Albu; Mert, AhmetNigella is a medicinal plant that has various pharmacological properties. It is widely used in folk medicine to treat many diseases in the world. It was aimed to investigate the essential oils Nigella sativa L. on two human melanoma cells (A-375 and A-2058 cell lines). In this study, two different cancer cell lines (A- 375and A-2058 cell lines) and one normal cell culture (vero cells) were used. Firstly, the non-toxic concentrations the essential oils of Nigella sativa L. were determined on normal cells (Vero cell line). And then, the cytotoxicity test were applied at these non-toxic concentrations. The cytotoxic activity of the essential oils of Nigella sativa L. on the cell lines was measured by MTT and Trypan blue assays. The chemical components analyses of the Nigella sativa L. oils were carried out by GC-MS. Medically important various components of Nigella sativa L. were determined in the GC-MS analysis. Some of these components are as follows; anethole (22.97%), thymoquinone (21.36%), a-thujene (6.22%), longifolene (5.76%), trans-isoeugenol (3.55%), carvacrol (2.23%). A total of 24 compounds were identified. The essential oils of Nigella sativa L. were found to inhibit the cell proliferation of human malignant melanoma cells. The IC50 values of the essential oils of Nigella sativa L. It were investigated as compared with a standard drug (methotrexate). Statistically significant decrease on the cell proliferations was found in the cells treated with the essential oils ofNigella sativa L. It was found to be non-toxic on normal cells (on Vero cell line) at the effective concentrations of the essential oils.While the non-toxic concentrations of essential oils on Vero cell line were found to be 12.5 ?g/ml, the effective concentrations for A-375 and A-2058 cell lines were as 1.56 ?g/ml and 3.12 ?g/ml, respectively. In conclusion, the essential oils of Nigella sativa L. were found to be significantly inhibited the cell proliferation on the human melanoma cells. The effect may arise from the components situated in the structure of Nigella sativa L. such asanethole, thymoquinone, a- thujene, longifolene, trans-isoeugenol and carvacrol. These components of this plant have been found promising in the treatment for human melanomas. But further studies, especially further animal studies should be needed to explain the action mechanisms of these components. © 2016 Certex, INCDTP-ICPI.Öğe New chalcone derivatives as effective against SARS-CoV-2 agent(Wiley-Hindawi, 2021) Duran, Nizami; Polat, M. Fatih; Aktas, Derya Anil; Alagoz, M. Abdullah; Ay, Emrah; Cimen, Funda; Tek, ErhanAims Flavonoids and related compounds, such as quercetin-based antiviral drug Gene-Eden-VIR/Novirin, inhibit the protease of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The alkylated chalcones isolated from Angelica keiskei inhibit SARS-CoV proteases. In this study, we aimed to compare the anti-SARS CoV-2 activities of both newly synthesized chalcone derivatives and these two drugs. Methods Determination of the potent antiviral activity of newly synthesized chalcone derivatives against SARS-CoV-2 by calculating the RT-PCR cycling threshold (C-t) values. Results Antiviral activities of the compounds varied because of being dose dependent. Compound 6, 7, 9, and 16 were highly effective against SARS-CoV-2 at the concentration of 1.60 mu g/mL. Structure-based virtual screening was carried out against the most important druggable SARS-CoV-2 targets, viral RNA-dependent RNA polymerase, to identify putative inhibitors that could facilitate the development of potential anti-coronavirus disease-2019 drug candidates. Conclusions Computational analyses identified eight compounds inhibiting each target, with binding affinity scores ranging from -4.370 to -2.748 kcal/mol along with their toxicological, ADME, and drug-like properties.Öğe RESISTANCE OF SARS COV-2 TO SEAWATER(Inst. Nat. Cercetare-Dezvoltare Text. Pielarie, 2022) Ay, Emrah; Duran, NizamiIn this study, it was aimed to comparatively investigate the grain properties of shoe upper leathers produced for different purposes. Thus, six different types of shoe upper leather (cracked, antique, patent, nappa, nubuck, printed) were provided from three different footwear companies. The tensile strength and elongation at break (TS EN ISO 3376), single and double edge tear strength (TS EN ISO 3377-1, TS EN ISO 3377-2), cracking and bursting resistance (TS 4137 EN ISO 3378, TS EN ISO 3379), flex resistance (TS EN ISO 5402-1) as well as dry and wet rubbing fastness tests (TS EN ISO 11640) were applied to leathers that have similar thicknesses. The results of the study gave information about the physical strength and product performances of different upper leather types. The data were evaluated comparatively and the effects of shoe upper leather types on quality and performance were evaluated. © 2022 Toate drepturile asupra acestei edi?ii sunt rezervate editorilor.Öğe Synergistic activities of the essential oils Hypericum perforatum with methotrexate on human breast cancer cell line MCF-7(Certex, INCDTP-ICPI, 2016) Duran, Gulay Gulbol; Duran, Nizami; Ay, Emrah; Kaya, Durmus Alpaslan; Kaya, Madalina Georgiana AlbuHypericum perforatum (Hypericaceae) is a perennial plant usually known as “Sarikantaron“ that has been reported to have various important biological activities.In this present study, it was aimed to (I) identify the components (ii) show the synergistic activities of the essential oils Hypericum perforatum with methotrexate on human breast cancer cell line MCF-7. A normal [Madin-Darby Bovine Kidney (MDBK) cell line] and a cancer cell line [Human breast adenocarcinoma cell line (MCF-7)] were used in this study. The cell culture were treated with various concentrations of Hypericum perforatum's oils. The cytotoxic activity of the essential oils of Hypericum perforatum on the cell lines was measured using the MTT method and the results were evaluated as IC50. In this study, the presence of trans-caryophyllene, germacrene-D, ?-pinene, trans-cadina-1,4-diene, ?- Selinene, caryophyllene oxide and ?-Selinene were identified as major constituents of Hypericum perforatum's oils. The essential oils of Hypericum perforatum also exhibited anticancer activities against MCF-7 cells. The IC50 values of the essential oils, MTX and the essentialoilsplus MTX weredetermined as 0.78, 6.25 and 0.195 ?g/ml, respectively, But, the essential oils of Hypericum perforatum was found to be non-cytotoxic for MDBK cells. In conclusion, the essential oils of Hypericum perforatum was chemically characterized and ?-muurolene, ?-cadinene, germacrene B, a-copaene, germacrene D, bicyclogermacrene, and (E)-caryophyllene were found to be major constituents. The essential oils of Hypericum perforatum possess significant in vitro anticancer potential. The essential oils of Hypericum perforatum with MTX found to be significant effective against breast cancer cells. Further studies especially in vivo anticancer properties of Hypericum perforatum should be searched. These compounds found to be very promising compounds in the treatment of cancer therapy. © 2016 Certex, INCDTP-ICPI.Öğe SYNERGISTIC EFFICACY OF EUCALYPTOL WITH ACYCLOVIR AGAINST HSV-2(Inst. Nat. Cercetare-Dezvoltare Text. Pielarie, 2022) Ay, Emrah; Duran, NizamiThe increasing drug resistance in herpes viruses in recent years brings with it new treatment approaches. In recent years, it has been tried to overcome drug resistance, especially with the use of herbal products in combination with existing drugs. In this study, we aimed to investigate the effectiveness of eucalyptol in combination with acyclovir. A Vero cell line was used for toxicity tests and viral culture isolation studies in the study. The non-toxic concentrations of eucalyptol and acyclovir were determined by the MTT method. Antiviral efficacy studies were performed within non-toxic concentrations. Antiviral activity was determined by calculating the IC50 values of the compounds against HSV-2. In addition, it was evaluated by the RT-PCR method. The 50% inhibitory concentration (IC50) and Fractional inhibitory concentration (FIC) index values determined during 24 hours and 48 hours of action showed that Eucalyptol exhibited a potent activity. This efficacy was found to be stronger when used in combination with acyclovir. These results show that the combination of Eucalyptol and acyclovir may be beneficial against resistant HSV infections. We suggest that the results of these studies, which are planned as in-vitro, be supported by in-vivo studies. © 2022 Toate drepturile asupra acestei edi?ii sunt rezervate editorilor.Öğe The therapeutic potential and molecular mechanism of Alpha-pinene, Gamma-terpinene, and P-cymene against melanoma cells(Cell Press, 2024) Acikgul, Funda Cimen; Duran, Nizami; Kutlu, Tuncer; Ay, Emrah; Tek, Erhan; Bayraktar, SuphiThe purpose of this study is to investigate the potential anticarcinogenic effects of three phytochemicals, namely Alpha-pinene (AP), Gamma-terpinene (GT), and P-cymene (PC), on melanoma cells (A2058 cell line). Additionally, the study aims to explore the synergistic activities of these phytochemicals with Dacarbazine, a chemotherapy drug. To understand the molecular mechanism involved in apoptosis induction in the A-2058 cell line, it was used AO/EB staining for apoptosis detection and cell cycle analysis, monitored through flow cytometry. It also determined the mRNA expression levels of different apoptosis-regulatory genes, including p53, Bax, NF-kB, Bcl-2, Bcl-xl, and caspase-3. The antitumor activities of these phytochemicals and their combinations were investigated in a subcutaneous mouse tumor model. The tumor diameter was 21.4 f 1.1 mm in the Dacarbazine treatment group and 42.4 f 3.1 mm in the control group. The antitumoral activities of AP and PC in the tumor model were similar to those of Dacarbazine. On the other hand, GT exhibited remarkable antitumoral activity, with a 1.75-fold reduction in tumor diameter compared to the Dacarbazine group. When different combinations of phytochemicals and Dacarbazine were used, the GT plus Dacarbazine treatment group was found to have a 3.5- fold reduction in tumor diameter compared to the Dacarbazine group. The tumor diameters in the Dacarbazine, AP plus GT, GT plus Dacarbazine, and AP plus Dacarbazine treatment groups were 21.4 f 1.1, 7.6 f 2.2, 8.6 f 0.5, and 6.2 f 1.9 mm, respectively.
