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Adi yalancıdarı ( Paspalum dilatatum Poir.) bitkisinin genç salkımlarından kallus oluşumu ve bitki rejenerasyonuna genotip ve 2, 4-D konsantrasyonunun etkileri üzerine bir araştırma
(2000) Can, Ersin; Çeliktaş, Nafiz; Hatipoğlu, Rüştü
This study was conducted to determine the effects of genotype and 2,4-D concentrations on the callus induction and plant regeneration from young inflorescences of dallisgrass ( Paspalum dilatatumPoir). Segments of young inflorescences from six different ecotypes of dallisgrass were cultured on MS-medium containing different con-centrations of 2,4-D ( 2, 4, 6, 8 and 10 mg/l). The results of the study showed that the ecotypes were significantly different in cal-lus induction ratio, callus weight per petri dish and plant regeneration from the young inflorescences. With respect to the ecotypes, callus induction ratio varied from 17.5 % to 65 %, callus weight from 75.25 to 365.1 mg/petri dish and number of regenerates per inflorescence segment from 0.775 to 1.612. The callus induction ratio, callus weight and regeneration ratio were also signifi-cantly influenced by the 2,4-D concentrations. The segments cultured on the MS medium containing 6 mg/l of 2,4-D gave the high-est values of callus induction ratio (74 %), callus weight (369.3 mg/petri dish) and regeneration ratio (2.094 regenerates perseg-ment).
Breaking seed dormancy of some annual Medicago and Trifolium species by different treatments
(2009) Can, Ersin; Çeliktaş, Nafız; Hatipoğlu, Rüştü; Avcı, Süleyman
The germination abilities of 14 annual legume species (six Medicago and eight Trifolium) were tested under three dormancy breaking treatments: In the first treatment, mechanical scarification plus sodium hypochlorite (MSS); in the second, chemical scarification with sulphuric acid (CSA); in the third, incubation in water bath at 90 °C (IWB) were used for breaking the dormancy. All Medicago species except for Medicago polymorpha showed improvement in germination rates using MSS. The seeds of M. polymorpha showed higher germination rates with CSA or IWB than MSS. For Trifolium species, results indicated that there was a great variability among species with regard to the treatments. Seeds of species showed a broad range of dormancy requirements for germination. The CSA treatment significantly increased the germination rates of Trifolium lappaceum, T. scabrum and T. strictum. On the other hand, the germination rates of T. spumosum, T. spadieceum and T. angustifolium were significantly improved by MSS. The seeds of T. badium showed higher germination rates with IWB than the other applications.
BREAKING SEED DORMANCY OF SOME ANNUAL MEDICAGO AND TRIFOLIUM SPECIES BY DIFFERENT TREATMENTS
(Soc Field Crop Sci, 2009) Can, Ersin; Celiktas, Nafiz; Hatipoglu, Ruestue; Avci, Sueleyman
The germination abilities of 14 annual legume species (six Medicago and eight Trifolium) were tested under three dormancy breaking treatments: In the first treatment, mechanical scarification plus sodium hypochlorite (MSS); in the second, chemical scarification with sulphuric acid (CSA); in the third, incubation in water bath at 90 degrees C (IWB) were used for breaking the dormancy. All Medicago species except for Medicago polymorpha showed improvement in germination rates using MSS. The seeds of M. polymorpha showed higher germination rates with CSA or IWB than MSS. For Trifolium species, results indicated that there was a great variability among species with regard to the treatments. Seeds of species showed a broad range of dormancy requirements for germination. The CSA treatment significantly increased the germination rates of Trifolium lappaceum, T scabrum and T strictum. On the other hand, the germination rates of T spumosum, T spadieceum and T angustifolium were significantly improved by MSS. The seeds of T badium showed higher germination rates with IWB than the other applications.
Comparison between a wild population and cultivar of common vetch (Vicia sativa L., Fabaceae) on cytological and agronomic characteristics
(Taylor & Francis Ltd, 2006) Celiktas, Nafiz; Can, Ersin; Hatipoglu, Rustu; Avci, Suleyman
The aim of this study was to compare a wild population of common vetch (Vicia saliva L.) collected from the Serinyol district of Antakya in the east Mediterranean coastal region of Turkey with a cultivar of common vetch (V saliva 'Anadolu'), on somatic chromosome number and important agronomic attributes. Somatic chromosome counts from material collected from the wild population of Vicia saliva L. were consistently 2n = 10, while the cultivar had 2n = 12. There were no significant differences between wild and cultivated cytotypes in plant height, seed number or pod number, although the wild cytotype had significantly more branches, smaller seeds, higher hardseededness and lower germination rates.
Comparison of shrub leaves in terms of chemical composition and nutritive value
(Kahramanmaraş Sütçü İmam Üniversitesi, 2019) Ertekin, İbrahim; Atış, İbrahim; Yılmaz, Şaban; Can, Ersin; Kızılşimşek, Mustafa
The goal of the present study was to compare the leaves of some shrubs in terms of chemical composition and nutritive value at the period of baby fruit. For this purpose, leaves of Quercus coccifera L (QC), Quercus branti Lindl (QB), Quercus vulcanica Boiss and Heldr. Ex Kotschy (QV), Phillyrea latifolia (L.) Salibs (PL), Styrax officinalis L (SO), Arbutus andrachne L (AA) and Olea europaea L (OE) plants were collected at the period of baby fruit. In this research, dry matter (DM), crude protein (CP), crude ash (CA), organic matter (OM), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), non-fiber carbohydrates (NFC), crude fat (CF) and condensed tannins (CT) was measured to determine the chemical compositions of leaves (P<0.01). In addition, dry matter intake (DMI), dry matter digestibility (DMD), relative feed value (RFV) and metabolic energy (MEADF) were calculated to evaluate the nutritive value of leaves. All investigated parameters were statistically different except for DM content of leaves. Results of current study showed that the SO had better nutritive value and chemical contents for livestock especially goats. It can be concluded that the CT content of SO can be examined at the flowering and/or earlier period in further studies.
Çukurova bölgesi doğal meralarında yaygın olarak bulunan sarı sakalotu ( Bothriochloa ischaemum L., keng ) bitkisinde sitilojik araştırmalar
(1999) Can, Ersin; Hatipoğlu, Rüştü
Bu araştırma, Çukurova bölgesi doğal mer'alarında yaygın olarak bulunan sarı sakalotu (Bothriochloa ischaemum (I) Keng) bitkisinde somatik kromozom sayısı ve kromozomların morfolojik özelliklerinin belirlenmesi amacıyla yapılmıştır. Araştırma sonuçları, incelenen 40 sarı sakalotu ekotipinden 39 tanesinin 2n=60, bir tanesinin ise 2n=50 kromozomlu olduğunu göstermiştir. Bitkide kromozom uzunluğunun 1.3006 pm ile 3.1294 pm arasında değiştiği ve kromozomların tümünün metasentrik olduğu saptanmıştır. Ayrıca, 2n=50 kromozomlu sitotipin 2 çift, 2n=60 kromozomlu sitotipin ise 3 çift satelitli kromozoma sahip olduğu ortaya çıkmıştır.
A detailed experimental knowledge on differentiation of nutritional quality depending on the phenological stages of native cool season grasses and satisfying the daily maintenance requirements of livestock
(Hatay Mustafa Kemal Üniversitesi, 2019) Çeliktaş, Nafiz; Can, Ersin; Kaya, Şerafeddin; Uygur, Veli
Aims: For a proper range management and livestock feeding operations need to detailed chemical analyses and knowledge on pasture composition. Methods and Results: Seven native cool-season grasses that are Festuca arundinaceae L., Brachypodium pinnatum (L.) Beauv., Phleum pretense L., Dactylis glomerata L., Bromus inermis Leyss., Lolium perenne L. and Hordeum bulbosum L. were evaluated for their nutritive value at five different phenological stages. The chemical composition, quality and therefore their feeding value so the animal performance are directly under control of the stage of plant phenology. Stage of plant maturity decreases dry matter digestibility, dry matter intake and protein content, but increases in lignocellulosic structure with the advance phenology of the whole investigated perennial species. Conclusions: The species which have thickand longer leaves, and higher leaf/stem ratio determined as the more digestible, and also the metabolizable energy of the fast growing ones was considerably higher. The vast majority of the minerals’ concentration was distinctly reduced at stem elongation to flowering and then increased remarkably from that point to developing seed stalks. The fast growing species accumulated the minerals more. Grass Tetany risk (K/(Ca+Mg)) of the investigated species increased remarkably at head emergence and flowering stage. Most of the species were detected within the boundaries of the recommended Ca/P ratio for diet. Significance and Impact of the Study: The native pastures seem to satisfy the daily maintenance requirements of most livestock in respect to most minerals without any supplementation via correct timing for feeding.
Doğal serin iklim buğdaygil yembitkilerinin bitki gelişme dönemlerine bağlı yem kalitesi değişimi ve çiftlik hayvanlarının günlük ihtiyacını karşılayabilme yetenekleri
(2019) Celıktas, Nafiz; Can, Ersin; Kaya, Şerafettin; Uygur, Veli
Amaç: Doğru bir mera yönetimi ve hayvan beslemeilkeleri açısından mera kompozisyonu üzerinde detaylıkimyasal inceleme ve yoğun bir bilgiye ihtiyaç vardır.Yöntem ve Bulgular: Mevcut araştırmada; doğal çokyıllık serin mevsim buğdaygil türleri, Festucaarundinaceae L., Brachypodium pinnatum (L.) Beauv.,Phleum pretense L., Dactylis glomerata L., Bromusinermis Leyss., Lolium perenne L. ve Hordeum bulbosumL. beş farklı bitki gelişim döneminde hayvan beslemekalitesi açısından değerlendirilmiştir. Bitki kimyasalkompozisyonu, kalite ve dolayısıyla besleme değerleriyani hayvan performansı bitki gelişim dönemlerindendoğrudan etkilenmektedir. İncelenen tüm türlerde kurumaddenin sindirilebilirliği, alımı ve protein içerikleri bitkiolgunlaşması ile birlikte azaltırken, lignifikasyonartmıştır.Genel Yorum: İnce uzun yapraklı ve yaprak/sap oranıyüksek olan türlerin sindirilebilirlik oranlarının dahayüksek oldukları belirlenmiştir. Hızlı büyüyen türlerde isemetabolize olabilir enerji içeriğinin yüksekliği dikkatedeğer bulunmuştur Mineral elementlerin büyükçoğunluğunun konsantrasyonu sapa kalkma veçiçeklenme dönemi arasında belirgin şekilde azalırken,bu dönemden başak/salkım çıkarma aşamasına kadarönemli bir artış saptanmıştır. Hızlı büyüyen türlerdemineral element birikimi de daha fazla gerçekleşmiştir.İncelenen türlerde çayır tetanisi riski (K/(Ca+Mg))çiçeklenme aşamasında belirgin bir şekilde artışgöstermiştir. Hesaplanan Ca/P oranının, tüm türler içinrasyonda olması önerilen sınırlar içinde bulunduğudeğerlendirilmiştir.Çalışmanın Önemi ve Etkisi: Araştırma bulgularıincelenen doğal mera bitkilerinin, çoğu mineral elementaçısından çiftlik hayvanlarının günlük gereksinimlerini, herhangi bir ilaveye gerek olmaksızın karşılayabilecekkapasitede olduklarını ortaya koymuştur.
Effect to the young inflorescence length on the callus formation and plant regeneration in yellow bluestem (Bothriochloa ischaemmum (L.) keng)
(TUBITAK, 2000) Can, Ersin; Çeliktaş, Nafiz; Hatipo?lu, Rüştü
This study was carried out to determine the effect of inflorescence length on callus induction and plant regeneration in yellow bluestem (Bothriochloa ischaemum (L) Keng). Inflorescences with different lengths (2-25 mm) from the in vitro regenerated plants of a Bothriochloa - ecotype with a good response to in vitro culture were cultured on LS-medium with addition 8 mg/1 dicamba. The results of the study showed that the rates of callus induction related to inflorescence length varied from 20.3% to 46.9%. The highest callus weight per inflorescence was obtained from the inflorescences with a length of 11-15 mm. Increasing the inflorescence length decreased the callus weight per inflorescence when inflorescences longer than 15 mm were used as explant. The highest rate of regeneration (10.860 plantlets per inflorescence) was obtained from the inflorescence with a length of 16-20 mm. Increasing the inflorescence length decreased the regeneration rate when inflorescences longer than 20 mm were used as expiant. It was concluded that inflorescences that are yellowish and 11-20 mm long can be used as explant for the establishment of the embryogenic callus cultures of Bothriochloa ischaemum.
THE EFFECTS OF CUTTING INTERVALS AND SEEDING RATES ON FORAGE YIELD AND QUALITY OF ALFALFA
(Soc Field Crop Sci, 2019) Atis, Ibrahim; Celiktas, Nafiz; Can, Ersin; Yilmaz, Saban
Understanding the effects of seeding rate and cutting intervals on dry matter yield and nutritive value may help to optimize of alfalfa production. The objective of this study was to investigate the optimum cutting interval and seeding rate of two cultivars of alfalfa (cv. Alsancak and cv. Nimet). Field experiments were carried out for 3 years in Mediterranean ecological conditions. The treatments were comprised of three cutting programs at 20, 30 and 40 days interval and two seeding rates (15 and 30 kg ha(-1)). The experiment was designed a split-split plot in a randomized complete block with 3 repetitions. The main plots were cutting intervals, sub-plots were seeding rates and sub -subplots were assigned as alfalfa cultivars. The results of the research showed that all properties inspected were significantly affected from the cutting intervals, the effect of the seeding rate and the cultivars were found to be not significant (except fresh forage yield). While the extended cutting intervals increased the forage yield, it reduced the forage quality. In terms of satisfactory forage yield and quality for similar ecological conditions, 30 days cutting interval and 15 kg ha(-1) seeding rate have been advisable.
Effects of cutting time and cutting height on the growth of dallisgrass (Paspalum dilatatum Poir.)
(2003) Can, Ersin; Çeliktaş, Nazif; Hatipoğlu, Rüştü; Yılmaz, Şaban; Avcı, Süleyman
The effects of three different cutting times and three different cutting heights on the hay yield and the root growth of dallisgrass (Paspalum dilatatum Poir.) were studied in 2001. The experiment was conducted as a pot trial and arranged in a split plot design with three replications. Main-plots were the cutting times (at 15 cm, 30 cm and 45 cm of plant height) and subplots were cutting heights (5 , 7.5 and 10 cm above ground). The results of the study showed that the highest tiller number, forage and hay yield and root weight were obtained from the plants harvested each time when they reached to 45 cm plant height at 5 cm cutting height. Also the earlier cutting plants gave the less yield than later cutting plants.
Effects of different media, type and concentrations of auxin on callus induction and plant regeneration from young inflorescences of yellow bluestem (Bothriochloa ischaemum (L.) Keng)
(TUBITAK, 2000) Can, Ersin; Hatipo?lu, Rüştü
This study was conducted to determine the effects of different media, auxin types and concentrations on callus induction and plant regeneration from young inflorescences of 15 ecotypes of yellow bluestem (Bothriochloa ischaemum). The young inflorescences were cultured on two different basal media (LS and SH) containing different concentrations (2, 4, 6, and 8 mg/l) of two different auxins (2, 4-D and Dicamba). The results showed that the ecotypes were significantly different from each other in callus induction rate, callus weight per dish and regenerate number per inflorescence segment. Depending on the ecotypes, mean callus induction rate varied from 9.7% 51.6%, mean callus weight per dish from 95.6 to 414.6 mg and number of regenerates per inflorescence segment from 0 to 2.2. LS medium resulted in better callus induction, and callus weight and regeneration than SH medium. While the media with 2,4-D produced higher callus induction and callus weight than those with dicamba. the media supplemented with dicamba produced more regenerates per inflorescence segment than those with 2,4-D. The highest value for both callus induction and callus weight was obtained at 4 mg/l 2,4-D concentration. However, the highest plant regeneration was recorded at 8 mg/l dicamba concentration.
Effects of different salt concentrations on the germination properties of Hungarian vetch (Vicia pannonica Crantz.) cultivars
(2018) Ertekin, İbrahim; Yılmaz, Şaban; Atak, Mehmet; Can, Ersin
This research was carried out in the Mustafa Kemal University, Faculty of Agriculture, Department of Field Crops. It was used two Hungarian vetch cultivars (Altinova-2002 and Anadolu Pembesi-2002) and five salt concentrations (unsalted/0, 50 mM, 100 mM, 150 mM, 200 mM) as treatment. In this experiment, it was estimated germination rate (%), germination index and mean germination time (day) by using germination parameters and was also measured root and shoot length (mm) and seedling fresh weight (g). Salt concentrations negatively affected evaluated features for both cultivars in the final of this research. As the salt concentrations increased, all investigated properties except mean germination time were observed to decrease. In terms of germination rate, germination index, root and shoot length and seedling fresh weight between cultivars, Altionva-2002 cultivar gave higher value than Anadolu Pembesi-2002 cultivar. When we examined Cultivar x dose interactions, all properties were most affected adverse in the Anadolu Pembesi x 200 mM interaction This research clearly showed that the cultivation of ungarian vetches of Anadolu Pembesi and Altınova-2002 will be affected in the soil that has been exposed to the problem of salinity.
Effects of genotype and 2,4,5-T concentrations on callus induction, shoot formation and plant regeneration from young inflorescences in orchardgrass (Dactylis glomerata L.)
(Wfl Publ, 2013) Atis, Ibrahim; Can, Ersin; Celiktas, Nafiz; Hatipoglu, Rustu
This study was carried out to determine the effects of MS medium supplemented with 2, 4, 6 and 8 mg l(-1) of 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) on young inflorescences of five ecotypes of orchardgrass (Dactylis glomerata L.). The results showed that there was a significant interaction between ecotypes and 2,4,5-T concentrations in all characteristics studied. Depending on the ecotypes, rate of callus induction (%), rate of shoot formation (%), callus weight (mg/per Petri dish) and number of regenerates per inflorescence segment varied and ranged 6.3-37.5%, 7.8-51.6%, 13.1-115.4 mg and 0.3-1.1, respectively. Rates of callus induction and shoot formation as well as callus weight were also significantly influenced by the 2,4,5-T concentrations. The explants cultured on MS medium containing 4 mg(-1) of 2,4,5-T showed highest callus induction rate of 53.8 % with callus weight of 170.4 mg/Petri dish and 1.6 shoots per explant. It was concluded that best regeneration from young inflorescence explant of orchardgrass (D. glomerata) could be obtained on MS medium containing 4 mg l(-1) of 2,4,5-T and the developed protocol will help in easy multiplication of orchard grass in future breeding studies.
Effects of genotype and concentration of 2,4-D on callus induction and plant regeneration from young inflorescences of dallisgrass (Paspalum dilatatum Poir)
(TUBITAK, 2000) Can, Ersin; Çeliktaş, Nafiz; Hatipo?lu, Rüştü
This study was conducted to determine the effects of genotype and 2,4-D concentrations on the callus induction and plant regeneration from young inflorescences of dallisgrass (Paspalum dilatatum Poir). Segments of young inflorescences from six different ecotypes of dallisgrass were cultured on MS-medium containing different concentrations of 2,4-D (2.4.6.8 and 10 mg/1). The results of the study showed that the ecotypes were significantly different in callus induction ratio, callus weight per petri dish and plant regeneration from the young inflorescences. With respect to the ecotypes, callus induction ratio varied from 17.5 % to 65 %, callus weight from 75.25 to 365.1 mg/petri dish and number of regenerates per inflorescence segment from 0.775 to 1.612. The callus induction ratio, callus weight and regeneration ratio were also significantly influenced by the 2.4-D concentrations. The segments cultured on the MS medium containing 6 mg/l of 2,4-D gave the highest values of callus induction ratio (74%), callus weight (369.3 mg/petri dish) and regeneration ratio (2.094 regenerates per segment).
Efficient somatic embryogenesis from immature inflorescences of dallisgrass (Paspalum dilatatum Poir.)
(Sejani Publ, 2006) Avci, Suleyman; Can, Ersin
Somatic embryogenesis was obtained from 0.5 cm long immature inflorescences of eight Turkish dallisgrass (Paspalum dilatatum Poir.) accessions on LS (Linsmaier and Skoog) medium supplemented with various concentrations of dicamba and picloram. It was observed that 7.5 mg l(-1) picloram in LS medium was more appropriate for somatic embryogenesis compared to any concentration of dicamba. No difficulty was experienced in converting somatic embryos on LS medium containing half concentrations of macro-, microelements and vitamins. All plantlets established and acclimatized successfully in greenhouse conditions.
FERMENTATION PROPERTIES AND NUTRITIVE VALUE OF SUNFLOWER ENSILED WITH DIFFERENT MIXING RATIOS OF SILAGE MAIZE, SWEET CORN AND SWEET SORGHUM
(2023) Ertekin, İbrahim; Can, Ersin
In recent years, with the effect of global warming, researches on the potential of plants that are more resistant to drought as forage crops and their ensiling properties have become increasingly widespread. Current study aimed to investigate the ability of ensiling, fermentation quality and nutritive value of a sunflower variety, which is more drought resistant than many forage crops, with silage corn, sweet corn and sweet sorghum at different mixing ratios. Plant species used as silage material in present study were grown simultaneously in separate plots. All plants were harvested by hand in the range of about 25-32% dry matter and these harvested crops were theoretically chopped with 2-3 cm electric shredding machine for silage. In addition to pure silages of all crop materials, mixed silages of 25%+75%, 50%+50% and 75%+25% were made in 3 replications. Besides some properties of silage beginning materials, some fermentation properties and feed quality parameters of resulted silages were investigated. As a result of this study, 50%+50% mixed silage of the sunflower variety used as silage material with maize, sweet corn and sweet sorghum increased the silage fermentation quality compared to the pure sunflower silages and improved the silage feed quality compared to the pure cereal (corn, sweet corn and sweet sorghum) silages.
PHYTOCHEMICAL SCREENING OF AMARANTH HERBAGE AND THEIR IMPRESSION ON THE ANTIOXIDANT ACTIVITY WITH AN ASSESSMENT OF FEEDING QUALITY
(Parlar Scientific Publications (P S P), 2021) Celiktas, Nafiz; Can, Ersin; Atis, Ibrahim; Durak, Derya
Multivariate relations of minerals, fatty acid profile, phenolic compounds and the antioxidant activity of Amaranthus species were studied in herbage, considering the livestock feed quality. Most of the mineral content of amaranth species detected as enough to satisfy the daily maintenance of any livestock. The Calf) ratio (4.5-11.0) of the hay was considerably high because of lower P accumulation. It was classified as Zn-poor in terms of the daily requirement of livestock but was so rich in respect of Cu and Fe. The unsaturation of the methyl esterified fat, which is predominantly linoleic acid (42.947.8 %), varied between 73.3-77.8 % depending on the species. The highest radical scavenging ability (4.58 mM Trolox kg(-1) DW as DPPH assay) was detected for the herbage of A. cruentus Zimbabwe. Protocatechuic acid predominant total phenolic compounds varied between 54.51-254.68 mg g(-1). Principal component analyses (PCA) between DPPH and the phytochemical compounds has classified the species with representing the total variation of 63.25% with the first two PC. According to partial least square regression (PLSR) the polyunsaturated fatty acids (PUFA), chlorogenic acid, Mg and. Zn are the major contributors of DPPH.
Plant density and mixture ratio effects on the competition between common vetch and wheat
(2012) Atis, Ibrahim; Kokten, Kagan; Hatipoglu, Rustu; Yilmaz, Saban; Atak, Mehmet; Can, Ersin
Mixtures of annual forage legumes with winter small grains for forage are practiced traditionally in the Mediterranean countries. The aim of the present study was to investigate the effects of variable seed mixture rates and different plant densities on the forage yield. The study also investigated the competition effects among mixture partners in the mixture of common vetch (Vicia sativa L.) and wheat (Triticum aestivium L.). Field trials were conducted at two locations (L1, Hatay and L2, Adana) during the 2008-09 and 2009-10 growing seasons. Three plant densities (200, 400, 600 plants m-2) and five seed mixture ratios (100 % V, 75% V + 25% W, 50% V + 50% W, 25% V + 75% W, 100 % W) of common vetch (V) and wheat (W) were used. The results of the study showed that increased plant density in the mixture resulted in increased dry matter yield per unit area. In addition, increased seed ratio of wheat in the mixture caused increased dry matter yield per unit area. Also, elevated dry matter yield resulted in increased crude protein yield. Land equivalent ratio (LER), aggressivity (A) and competitive ratio (CR) indexes showed that common vetch was the dominant species in all common vetch-wheat mixtures.
Production of dihaploids in durum wheat using Imperata cylindrica L. mediated chromosome elimination
(2015) Çeliktaş, Nafiz; Tiryakioğlu, Murat; Can, Ersin; Kutlay, Duygu; Hatipoğlu, Rüştü
The possibility of obtaining dihaploid durum wheat ( Triticum durum L.) plants by interspecifc hybridization with Imperata cylindrica L. was studied. Ten local varieties and 1 commercial durum wheat variety (Triticum durum L., 2n = 4x = 28; AABB) were used as maternal lines. Imperata cylindrica L. (2n = 2x = 20) ecotypes sampled from diferent districts of Hatay were used as the pollen source. Isolated embryos were placed in an MS medium for plant regeneration 14 days afer the interspecifc hybridization. Te highest average seed setting, embryo formation, and dihaploid plant regeneration for the diferent hybrid combinations were determined as 31.1%, 15.5%, and 11.5%, respectively. Cytological observations revealed that all the regenerants had 2n = 2x = 14 chromosomes.

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