Bağlarda yeni saptanan virüslerin Hatay ve Tekirdağ ili bağ alanlarında PCR yöntemiyle belirlenmesi ve moleküler karakterizasyonu
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Tarih
2019
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Science and Technology Publishing (TURSTEP)
Erişim Hakkı
info:eu-repo/semantics/openAccess
Attribution-NonCommercial 3.0 United States
Attribution-NonCommercial 3.0 United States
Özet
Son yıllarda yeni nesil sekans analiz tekniklerinin yaygın olarak kullanılması bağlarda ve diğer bitki
türlerinde etiyolojisi bilinmeyen birçok virüs hastalığının etmeninin belirlenmesini sağlamıştır..
Bağlarda bu teknoloji ile saptanan yeni virüslerden en önemlileri Grapevine Pinot Gris Virus
(GPGV), Grapevine Syrah Virus-1 (GSyV-1), Grapevine Red-blotch associated Virus (GRBaV) ve
Grapevine Roditis Leaf Discoloration Virus (GRLDaV)’dür. Bu çalışmanın amacı Tekirdağ ve
Hatay ili bağ alanlarında GPGV, GSyV-1, GRBaV ve GRLDaV varlığının PCR ve DNA dizileme
yöntemleriyle saptanması ve karakterizasyonlarının yapılmasıdır. Bu çalışma kapsamında virüs
benzeri simptom gösteren omcaların yanı sıra simptomsuz omcalardan da örnekler alınmış ve toplam
olarak Tekirdağ ilinden 191, Hatay ilinden ise 111 örnek toplanmıştır. Tekirdağ ilinden toplanan bağ
örneklerinde %43,62 oranında GPGV, %1,04 oranında GSyV1 saptanmıştır. Hatay ilinden toplanan
örneklerde ise sadece %0,9 oranında GSyV1 saptanmış ve testlenen örnekler GPGV, GRBaV,
GRLDaV açısından temiz bulunmuştur. Tekirdağ örneklerinin GPGV için yapılan RT-PCR
analizlerinde kısmi kılıf protein, hareketlilik, ve replikaz genlerini çoğaltan primerler kullanıldığında
sırasıyla 411 bp, 302 bp ve 618 bp büyüklüğünde PCR ürünleri elde edilmiştir Bu ürünlerin doğrudan
iki yönlü sekans analizi sonucunda ve her üç gen bölgesinin de nükleotid dizilimlerinin gen
bankasında kayıtlı farklı GPGV izolatları ile yüksek oranda homoloji gösterdiği saptanmıştır.
The improvements on the next generation sequencing or high-trough output technologies allowed the discovery of several unknown viruses in grapevines and also in other plants . The most important new emerging grapevine viruses were identified as Grapevine pinot gris virus (GPGV), Grapevine syrah virus 1 (GSyV-1), Grapevine red blotch-associated virus (GRBaV) and Grapevine roditis leaf discoloration virus (GRLDaV). The aim of the present study was to investigate the occurrence and characterization of these viruses in Tekirdag and Hatay viticulture production areas by PCR and DNA sequencing analyses. Totally 191 and 111 grapevine samples showing both virus-like symptoms and symptomless were collected from Tekirdağ and Hatay provinces, respectively. Among the tested samples GPGV and GSyV-1 were detected in both local and imported cultivars by the infection rate of 43.62 % and 1.04% in Tekirdağ , respectively. In Hatay provice, only GSyV1 was detected by the infection rate of 0.9 % and all tested samples were negative for GPGV, GRBaV, GRLDaV. RT-PCR results showed that DNA fragments of 411 bp, 302 bp and 618 bp corresponding to the part of the coat protein (CP), movement protein (MP) and the replicase genes of GPGV were successfully amplified in Tekirdağ samples. All PCR products of GPGV were directly sequenced on both strands. All the nucleotide sequences of CP, MP and 5’ UTR and Nterminus of replicase genes shared the highest sequence identity with different GPGV isolates deposited in Genbank
The improvements on the next generation sequencing or high-trough output technologies allowed the discovery of several unknown viruses in grapevines and also in other plants . The most important new emerging grapevine viruses were identified as Grapevine pinot gris virus (GPGV), Grapevine syrah virus 1 (GSyV-1), Grapevine red blotch-associated virus (GRBaV) and Grapevine roditis leaf discoloration virus (GRLDaV). The aim of the present study was to investigate the occurrence and characterization of these viruses in Tekirdag and Hatay viticulture production areas by PCR and DNA sequencing analyses. Totally 191 and 111 grapevine samples showing both virus-like symptoms and symptomless were collected from Tekirdağ and Hatay provinces, respectively. Among the tested samples GPGV and GSyV-1 were detected in both local and imported cultivars by the infection rate of 43.62 % and 1.04% in Tekirdağ , respectively. In Hatay provice, only GSyV1 was detected by the infection rate of 0.9 % and all tested samples were negative for GPGV, GRBaV, GRLDaV. RT-PCR results showed that DNA fragments of 411 bp, 302 bp and 618 bp corresponding to the part of the coat protein (CP), movement protein (MP) and the replicase genes of GPGV were successfully amplified in Tekirdağ samples. All PCR products of GPGV were directly sequenced on both strands. All the nucleotide sequences of CP, MP and 5’ UTR and Nterminus of replicase genes shared the highest sequence identity with different GPGV isolates deposited in Genbank
Açıklama
Anahtar Kelimeler
Vitis vinifera, GPGV, GSyV-1, GRBaV, GRLDaV, Hatay, Tekirdağ, Vitis vinifera, GPGV, GSyV-1, GRBaV, GRLDaV, Turkey
Kaynak
Türk Tarım - Gıda Bilim ve Teknoloji dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
7
Sayı
5
Künye
Kocabağ, H. D., Çağlayan, K., & Gazel, M. (2019). Molecular Detection and Characterization of New Emerging Viruses by PCR Analysis in Hatay and Tekirdag Vineyards. Turkish Journal of Agriculture-Food Science and Technology, 7(5), 789-798.
