IN VITRO STUDIES ON THE PROTECTIVE EFFECT OF TANNIC ACID OF U87 CELLS INDUCED BY BETA-AMYLOID
Yükleniyor...
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dokuz Eylul Univ Inst Health Sciences
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Background: While the prevalence of Alzheimer's disease continues to increase throughout the twentieth century, the cause and pathology of the disease are not well understood and scientists are seeking treatments for the disease. Tannic acid can be used effectively to treat Alzheimer's disease and seems to be one of the alternative therapeutic strategies in medicine. In this study, we aimed to investigate the effects of tannic acid on the U87 (human astrocytoma cell line) treated with amyloid-beta (A beta), which is the Alzheimer's disease (AD) model cell line. Materials and Methods: In the study; three groups were formed as the control group, the A beta group, and the A beta + tannic acid group obtained by adding tannic acid to the A beta group. Firstly, the cytotoxic potential of TA in U87 cells was investigated by the colorimetric MTT (3-4,5-dimethyl-thiazolyl-2,5 diphenyltetrazolium bromide) test. To determine the antioxidant status in the cell line treated with tannic acid, to examine the effects of total oxidant status (TOS), superoxide dismutase (SOD), total antioxidant status (TAS) and catalase (CAT) activities, were measured by the ELISA method. Results: In our study, the viability and proliferation of the cell decreased in U87 cells treated with amyloidB compared to the control group, but tannic acid increased cell viability and proliferation when compared with the group treated with amyloid-B. When compared to the control group, the TAS, SOD, and CAT levels were significantly decreased in the U87 cell line exposed to A beta; TOS levels were found to increase significantly. Conclusions: In in vitro experiments, we determined that tannic acid has a protective effect by increasing antioxidant parameters in the amyloid beta-induced cell line.
Açıklama
Anahtar Kelimeler
Tannic acid, Amyloid-beta, U87, Alzheimer's
Kaynak
Journal of Basic and Clinical Health Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
5
Sayı
3
