The Effects on Post-Thaw Sperm Quality and Nuclear DNA Integrity of Supplementation of Low-Density Lipoprotein to Freezing Extender in the Mouse
Yükleniyor...
Dosyalar
Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Mice are an important research tool for genetic and molecular biology, allowing researchers to explore a variety of human illness models. Egg yolk is a common component of semen extenders for domestic animals and low-density lipoproteins (LDL) from egg yolk have some cryoprotective properties. This study aimed to investigate sperm quality characteristics and nuclear DNA integrity after post-thawing in an extender (18% raffinose + 3% skim milk) supplemented with different concentrations of LDL (2.5%, 5.0%, 7.5%, or 10%) in mice. 18% Raffinose+3% skim milk extender was used as a control group without LDL. CD-1 mice were used in the study, and semen was collected from the cauda epididymis and diluted with the extender. The straws were then frozen and thawed to evaluate progressive motility, viability, plasma membrane (HOST), acrosome, and nuclear DNA integrity parameters. Fresh sperm had the highest progressive motility, viability, plasma membrane integrity, and longevity (endurance) of progressive motility for 4 h in HTF solution. The greatest spermatologic results, including nuclear DNA integrity, were determined in fresh sperm (p<0.05). The greatest post-thaw progressive motility (56.7±1.3%), viability (11.6±2.9%), membrane integrity (63.0±2.7%), and longevity at the end of the 4 h (31.3±2.1%) were found in the group with supplementation of 2.5% LDL compared with the control group. The lowest progressive motility, viability, and plasma membrane integrity were determined in the 10% LDL group (p<0.05). The cryopreservation process increased the rates of fragmented DNA in all tested LDL and control groups compared with fresh sperm (p<0.05). There was no statistical difference between the control and experimental groups in terms of nuclear DNA damage after freezing and thawing (p<0.05). It was concluded that the addition of 2.5% LDL to the extender improved the spermatological quality parameters after freezing and could be used in the freezing and preservation of mouse sperm as it showed a higher protective effect against cold shock.
Açıklama
Anahtar Kelimeler
Mouse sperm, Low Density Lypoprotein, Sperm analysis
Kaynak
Van Veterinary Journal
WoS Q Değeri
Scopus Q Değeri
Cilt
35
Sayı
1
