The Effects on Post-Thaw Sperm Quality and Nuclear DNA Integrity of Supplementation of Low-Density Lipoprotein to Freezing Extender in the Mouse

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dc.contributor.authorYıldız, Cengiz
dc.contributor.authorErol, İbrahim
dc.date.accessioned2024-09-19T16:25:00Z
dc.date.available2024-09-19T16:25:00Z
dc.date.issued2024
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractMice are an important research tool for genetic and molecular biology, allowing researchers to explore a variety of human illness models. Egg yolk is a common component of semen extenders for domestic animals and low-density lipoproteins (LDL) from egg yolk have some cryoprotective properties. This study aimed to investigate sperm quality characteristics and nuclear DNA integrity after post-thawing in an extender (18% raffinose + 3% skim milk) supplemented with different concentrations of LDL (2.5%, 5.0%, 7.5%, or 10%) in mice. 18% Raffinose+3% skim milk extender was used as a control group without LDL. CD-1 mice were used in the study, and semen was collected from the cauda epididymis and diluted with the extender. The straws were then frozen and thawed to evaluate progressive motility, viability, plasma membrane (HOST), acrosome, and nuclear DNA integrity parameters. Fresh sperm had the highest progressive motility, viability, plasma membrane integrity, and longevity (endurance) of progressive motility for 4 h in HTF solution. The greatest spermatologic results, including nuclear DNA integrity, were determined in fresh sperm (p<0.05). The greatest post-thaw progressive motility (56.7±1.3%), viability (11.6±2.9%), membrane integrity (63.0±2.7%), and longevity at the end of the 4 h (31.3±2.1%) were found in the group with supplementation of 2.5% LDL compared with the control group. The lowest progressive motility, viability, and plasma membrane integrity were determined in the 10% LDL group (p<0.05). The cryopreservation process increased the rates of fragmented DNA in all tested LDL and control groups compared with fresh sperm (p<0.05). There was no statistical difference between the control and experimental groups in terms of nuclear DNA damage after freezing and thawing (p<0.05). It was concluded that the addition of 2.5% LDL to the extender improved the spermatological quality parameters after freezing and could be used in the freezing and preservation of mouse sperm as it showed a higher protective effect against cold shock.en_US
dc.identifier.doi10.36483/vanvetj.1417880
dc.identifier.endpage100en_US
dc.identifier.issn2149-3359
dc.identifier.issn2149-8644
dc.identifier.issue1en_US
dc.identifier.startpage94en_US
dc.identifier.trdizinid1233211en_US
dc.identifier.urihttps://doi.org/10.36483/vanvetj.1417880
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1233211
dc.identifier.urihttps://hdl.handle.net/20.500.12483/16128
dc.identifier.volume35en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofVan Veterinary Journalen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMouse spermen_US
dc.subjectLow Density Lypoproteinen_US
dc.subjectSperm analysisen_US
dc.titleThe Effects on Post-Thaw Sperm Quality and Nuclear DNA Integrity of Supplementation of Low-Density Lipoprotein to Freezing Extender in the Mouseen_US
dc.typeArticleen_US

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