Protective Effect of Vitamin C (Ascorbic Acid) Supplementation on Post-Thaw Motility and Fertility of Cryopreserved Rainbow Trout (Oncorhynchus mykiss) Sperm
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Date
2020
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info:eu-repo/semantics/openAccess
Abstract
Ascorbic acid (Vitamin C) is one of the important antioxidants, which naturally present in seminal\rplasma of fish. On the other hand, whether its effect may improve sperm quality following\rcryopreservation process still remains its uncertainty. Thus, the present study aimed to analyse the\reffect of different extenders supplemented with different ascorbic acid concentrations on post-thaw\rmotility and fertility of frozen-thawed rainbow trout (Oncorhynchus mykiss) sperm. Selected sperm\rsamples were pooled and diluted at 1:3 ratios with two different extenders (E) composing such as (E-1)\r300 mM glucose, 10% egg yolk and 10% DMSO and (E-2) 0.6 mM sucrose and 10% DMSO. Each\rextender was supplemented with vitamin C at 1, 5 and 10 mM concentrations. Following dilution,\rthe sperm was loaded into 0.25 ml straws and frozen in liquid nitrogen vapour. The straws were then\rplunged into liquid nitrogen for storage. Fertilization was carried out using the dry fertilization\rtechnique. Highest post-thaw motility (50±5.77) and fertilization (56±1.00) results were obtained\rwith the extender-1 (E-1) containing 10% DMSO concentration. In conclusion, the present study\rindicated that addition of ascorbic acid to the extenders improved rainbow trout sperm motility\rresulting higher fertilization of the eggs
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Türk Tarım - Gıda Bilim ve Teknoloji dergisi
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Volume
8
Issue
6
