Protective Effect of Vitamin C (Ascorbic Acid) Supplementation on Post-Thaw Motility and Fertility of Cryopreserved Rainbow Trout (Oncorhynchus mykiss) Sperm

Basit Öğe Kaydı
dc.contributor.authorYavuz, Uğur
dc.contributor.authorBozkurt, Yusuf
dc.date.accessioned2024-09-19T16:22:23Z
dc.date.available2024-09-19T16:22:23Z
dc.date.issued2020
dc.departmentHatay Mustafa Kemal Üniversitesien_US
dc.description.abstractAscorbic acid (Vitamin C) is one of the important antioxidants, which naturally present in seminal\rplasma of fish. On the other hand, whether its effect may improve sperm quality following\rcryopreservation process still remains its uncertainty. Thus, the present study aimed to analyse the\reffect of different extenders supplemented with different ascorbic acid concentrations on post-thaw\rmotility and fertility of frozen-thawed rainbow trout (Oncorhynchus mykiss) sperm. Selected sperm\rsamples were pooled and diluted at 1:3 ratios with two different extenders (E) composing such as (E-1)\r300 mM glucose, 10% egg yolk and 10% DMSO and (E-2) 0.6 mM sucrose and 10% DMSO. Each\rextender was supplemented with vitamin C at 1, 5 and 10 mM concentrations. Following dilution,\rthe sperm was loaded into 0.25 ml straws and frozen in liquid nitrogen vapour. The straws were then\rplunged into liquid nitrogen for storage. Fertilization was carried out using the dry fertilization\rtechnique. Highest post-thaw motility (50±5.77) and fertilization (56±1.00) results were obtained\rwith the extender-1 (E-1) containing 10% DMSO concentration. In conclusion, the present study\rindicated that addition of ascorbic acid to the extenders improved rainbow trout sperm motility\rresulting higher fertilization of the eggsen_US
dc.identifier.doi10.24925/turjaf.v8i6.1348-1352.3371
dc.identifier.endpage1352en_US
dc.identifier.issn2148-127X
dc.identifier.issue6en_US
dc.identifier.startpage1348en_US
dc.identifier.trdizinid525440en_US
dc.identifier.urihttps://doi.org/10.24925/turjaf.v8i6.1348-1352.3371
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/525440
dc.identifier.urihttps://hdl.handle.net/20.500.12483/15819
dc.identifier.volume8en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleProtective Effect of Vitamin C (Ascorbic Acid) Supplementation on Post-Thaw Motility and Fertility of Cryopreserved Rainbow Trout (Oncorhynchus mykiss) Spermen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
15819.pdf
Boyut:
392.43 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu