Sığırlarda GSBL ve AmpC sentezleyen Escherichia coli'nin prevalansı ve karakterizasyonu
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Tarih
2017
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info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, Hatay ilinde sığırlarda genişlemiş spektrumlu ?-laktamaz (GSBL) ve/veya plazmid aracılı AmpC tip ?-laktamaz (AmpC) sentezleyen E. coli'nin prevalansının belirlenmesi hedeflendi. Bu amaçla, sağlıklı görünüşlü 312 sığırdan rektal sıvab örneği toplandı. Fenotipik GSBL sentezi disk kombinasyon metodu ve çift disk sinerji testi ile ?-laktamaz (blaCTX-M, blaCMY-2, blaSHV, blaOXA ve blaTEM) ve plazmid aracılı kinolon direnç (PAKD) genleri (qnrA, qnrB, qnrS ve aac(6')-Ib) polimeraz zincir reaksiyonu (PZR) ve daha sonra sekans analizi ile araştırıldı. Ayrıca, GSBL ve/veya AmpC sentezleyen E. coli izolatlarının antimikrobiyal duyarlılıkları disk difüzyon metodu ile filogenetik gruplarının belirlenmesi ise PZR ile incelendi. Yirmialtı izolat (%8.3) GSBL üretimi yönünden fenotipik testlerle pozitif bulundu. Bu izolatlarda blaCTX-M-15 (n= 12), blaCTX-M-1 (n= 11), blaCTX-M-3 (n= 2) ve blaCMY-2 (n=1) genleri belirlendi. PAKD genleri 11 (%42.3) ESBL sentezleyen E. coli izolatında saptandı ve bu izolatlar sadece aac(6')-Ib-cr ve qnrS1 genleri yönünden pozitif bulundu. İzolatlarının 22'si (%84.6) çoğul direnç fenotipi gösterdi. GSBL/AmpC sentezleyen E. coli izolatlarının B1 (%50), A (%34.6) ve D (%15.4) filogruplarına ait olduğu görüldü. Bu çalışma ile, ilk kez Türkiye'de sığırlarda CTX-M-15, CTX-M-3, CTX-M-1 ve CMY-2 tip ?-laktamaz sentezleyen E. coli varlığı ve bu izolatların bazılarında aac(6')-Ib-cr ve qnrS1 genlerinin birlikte bulunduğu gösterilmiştir.
In this study, it was aimed to determine the prevalence of extended spectrum ?-lactamase (ESBL) and/or AmpC type ?-lactamase (AmpC) producing Escherichia coli from cattle in Hatay. For this purpose, 312 rectal swabs samples were collected from apparently healthy cattle. ESBL production was phenotypically investigated by disc combination method and double disc synergism test and ?-lactamase genes (blaCTX-M, blaCMY-2, blaSHV, blaOXA, and blaTEM) and plasmid mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS and aac(6')-Ib) were screened by polymerase chain reaction (PCR) and subsequent sequence analysis. Antimicrobial susceptibility of the isolates were determined using disc diffusion method and their phylogenetic groups were also searched by PCR. Twenty six (8.3%) isolates were found to be ESBL producer by phenotypic tests. The following ESBL/AmpC genes were detected: blaCTX-M-15 (n= 12), blaCTX-M-1 (n=11), blaCTX-M-3 (n=2), and blaCMY-2 (n=1). PMQR genes were detected in 11 (42.3%) ESBL producing E. coli isolates and these isolates were only positive for aac(6')-Ib-cr and qnrS1 genes. Twenty two (84.6%) of the isolates exhibited multidrug resistance (MDR) phenotype. ESBL/AmpC producing E. coli isolates were observed to be belonged to B1 (50%), A (34.6%) and D (15.4%) phylogroups. This study was the first to describe the presence of CTX-M-15, CTX-M-3, CTX-M-1 and CMY-2 producing E. coli in cattle in Turkey and the co-existence of aac(6')-Ib-cr and qnrS1 genes in some isolates.
In this study, it was aimed to determine the prevalence of extended spectrum ?-lactamase (ESBL) and/or AmpC type ?-lactamase (AmpC) producing Escherichia coli from cattle in Hatay. For this purpose, 312 rectal swabs samples were collected from apparently healthy cattle. ESBL production was phenotypically investigated by disc combination method and double disc synergism test and ?-lactamase genes (blaCTX-M, blaCMY-2, blaSHV, blaOXA, and blaTEM) and plasmid mediated quinolone resistance (PMQR) genes (qnrA, qnrB, qnrS and aac(6')-Ib) were screened by polymerase chain reaction (PCR) and subsequent sequence analysis. Antimicrobial susceptibility of the isolates were determined using disc diffusion method and their phylogenetic groups were also searched by PCR. Twenty six (8.3%) isolates were found to be ESBL producer by phenotypic tests. The following ESBL/AmpC genes were detected: blaCTX-M-15 (n= 12), blaCTX-M-1 (n=11), blaCTX-M-3 (n=2), and blaCMY-2 (n=1). PMQR genes were detected in 11 (42.3%) ESBL producing E. coli isolates and these isolates were only positive for aac(6')-Ib-cr and qnrS1 genes. Twenty two (84.6%) of the isolates exhibited multidrug resistance (MDR) phenotype. ESBL/AmpC producing E. coli isolates were observed to be belonged to B1 (50%), A (34.6%) and D (15.4%) phylogroups. This study was the first to describe the presence of CTX-M-15, CTX-M-3, CTX-M-1 and CMY-2 producing E. coli in cattle in Turkey and the co-existence of aac(6')-Ib-cr and qnrS1 genes in some isolates.
Açıklama
Anahtar Kelimeler
Veterinerlik
Kaynak
Kafkas Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
23
Sayı
1
