Yazar "Cuming, AC" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim
    Öğe
    Identification of germin isoforms in wheat callus
    (Akademiai Kiado Zrt, 2004) Caliskan, M; Turet-Sayin, M; Turan, C; Cuming, AC
    Application of auxin causes cells of the wheat embryo to undergo dedifferentiation and form callus tissue. The synthesis of germin isoforms (G, G' and psiG) has been selectively associated with different phases of embryonic development and callus formation. G and G' germin isoforms are found to be associated with germination. psiG is an embryogenesis related germin isoform. It is clearly important to distinguish whether callus induction caused a precocious initiation of embryonic maturation-specific pseudogermin synthesis, or whether it effected a switch in the pattern of protein synthesis; eliciting accumulation of the normally germination-related germin proteins. To distinguish between these alternatives, it is necessary to identify the germin protein isoforms accumulated during callus formation. Following the electrophoretic separation of callus protein extracts, the position of germin isomers were detected by Western-blotting. The immunoreactive polypeptides accumulating in callus tissue were determined to be exclusively germin, rather than psiG.
  • Küçük Resim
    Öğe
    Isolation and localization of new germination-related sequences from wheat embryos
    (Springer Singapore Pte Ltd, 2003) Caliskan, M; Bashiardes, S; Ozcan, B; Cuming, AC
    Subtractive library hybridization was used to isolate the cDNA clones that corresponded to the transcripts that were specifically up-regulated during wheat embryo germination. The clones with numbers 5, 6, 7, 8, 24, and 26 appeared to be more abundant in germinating wheat embryos. Among the isolated clones, we identified four new members of the wheat germin gene family. We also identified two novel sequences which exhibited distinct germination up-regulation, and displayed characteristic spatial patterns of expression. One of these, represented by clone pSB10, was principally expressed in the root tissue of germinating embryos. The second was represented by the pSB7 clone and was expressed in both the root and shoot primordia of the embryonic axis, as well as within the coleoptile.
  • [ N/A ]
    Öğe
    Localization of germin genes and their products in developing wheat coleoptiles
    (Springer-Verlag Singapore Pte Ltd, 2004) Caliskan, M; Ozcan, B; Turan, C; Cuming, AC
    Germination is a process which characterized with nescient synthesis of genes. Among the genes synthesized during the germination of wheat embryos, germin genes, proteins and their enzymatic activity were defined. Germin is a water soluble homopentameric glycoprotein which is remarkable resistant to degradation by a broad range of proteases including pepsin. Germin proteins found to have strong oxalate oxidase activity which produces hydrogen peroxide by degrading oxalic acid. The current study, aimed to localize the germin genes, proteins and enzymatic activities in developing coleoptiles which is a rapidly growing protective tissue of leaf primordium and shoot apex. Non-radioactively labeled germin riboprobes were employed to localize germin mRNAs in situ. FITC (Fluorescein isothiocyanate) and alkaline phosphatase linked anti-germin antibodies were used to localize germin proteins under the fluorescence and light microscopy and finally germin enzymatic activity was localized by using appropriate enzyme assay. The results revealed that in coleoptiles germin genes, proteins and their enzymatic activity were predominantly associated with the cells of epidermis and vascular bundle sheath cells.